Reyes-Corona, David Vázquez-Hernández, Nallely Escobedo, Lourdes Orozco-Barrios, Carlos E. Ayala-Davila, Jose Moreno, Mario Gil Amaro-Lara, Miriam E. Flores-Martinez, Yazmin M. J. Espadas-Alvarez, Armando Fernandez-Parrilla, Manuel A. Gonzalez-Barrios, Juan A. Gutierrez-Castillo, ME González-Burgos, Ignacio Martinez-Fong, Daniel NRTN gene transfection increases TH(+) fibers in the striatum of rats with chronic 6-OHDA lesion. <p><b>A.</b> Representative micrographs of TH immunohistochemistry. Headings = Anterior-posterior coordinates of Paxinos and Watson Rat Atlas [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0188239#pone.0188239.ref054" target="_blank">54</a>]. 24WLUT = Untransfected rats with 24 weeks of 6-OHDA lesion. 12WL4WT = 12 weeks after lesion and 4 weeks after transfection, 12WL12WT = 12 weeks after lesion and 12 weeks after transfection. The scale bar of 1 mm is for slices at different levels and of 100 μm for details. <b>B.</b> Densitometry of TH(+) relative area. 4WT and 12 WT = 4 and 12 weeks after transfection. 24WL = untransfected rats with 24 weeks of lesion. 12WL = 12 weeks after lesion. The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. One-way ANOVA and Newman-Keuls post-test. * <i>P</i> < 0.001, ** <i>P</i> < 0.0001. ns = no statistical significance, <i>P</i> > 0.05.</p> postsynaptic;NRTN causes presynaptic;neurotrophic;nigrostriatal dopaminergic system;NTS-polyplex nanoparticles system;Neurotensin receptor type 1 levels;6- hydroxydopamine lesion;dopaminergic neurons;striatum;OHDA;neurite;striatal dendritic spines;substantia nigra;striatal medium spiny neurons;recovery;TH;overexpression 2017-11-27
    https://plos.figshare.com/articles/figure/NRTN_gene_transfection_increases_TH_fibers_in_the_striatum_of_rats_with_chronic_6-OHDA_lesion_/5635864
10.1371/journal.pone.0188239.g003