10.1371/journal.pone.0188239.g003 David Reyes-Corona David Reyes-Corona Nallely Vázquez-Hernández Nallely Vázquez-Hernández Lourdes Escobedo Lourdes Escobedo Carlos E. Orozco-Barrios Carlos E. Orozco-Barrios Jose Ayala-Davila Jose Ayala-Davila Mario Gil Moreno Mario Gil Moreno Miriam E. Amaro-Lara Miriam E. Amaro-Lara Yazmin M. Flores-Martinez Yazmin M. Flores-Martinez Armando J. Espadas-Alvarez Armando J. Espadas-Alvarez Manuel A. Fernandez-Parrilla Manuel A. Fernandez-Parrilla Juan A. Gonzalez-Barrios Juan A. Gonzalez-Barrios ME Gutierrez-Castillo ME Gutierrez-Castillo Ignacio González-Burgos Ignacio González-Burgos Daniel Martinez-Fong Daniel Martinez-Fong NRTN gene transfection increases TH(+) fibers in the striatum of rats with chronic 6-OHDA lesion. Public Library of Science 2017 postsynaptic NRTN causes presynaptic neurotrophic nigrostriatal dopaminergic system NTS-polyplex nanoparticles system Neurotensin receptor type 1 levels 6- hydroxydopamine lesion dopaminergic neurons striatum OHDA neurite striatal dendritic spines substantia nigra striatal medium spiny neurons recovery TH overexpression 2017-11-27 18:31:25 Figure https://plos.figshare.com/articles/figure/NRTN_gene_transfection_increases_TH_fibers_in_the_striatum_of_rats_with_chronic_6-OHDA_lesion_/5635864 <p><b>A.</b> Representative micrographs of TH immunohistochemistry. Headings = Anterior-posterior coordinates of Paxinos and Watson Rat Atlas [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0188239#pone.0188239.ref054" target="_blank">54</a>]. 24WLUT = Untransfected rats with 24 weeks of 6-OHDA lesion. 12WL4WT = 12 weeks after lesion and 4 weeks after transfection, 12WL12WT = 12 weeks after lesion and 12 weeks after transfection. The scale bar of 1 mm is for slices at different levels and of 100 μm for details. <b>B.</b> Densitometry of TH(+) relative area. 4WT and 12 WT = 4 and 12 weeks after transfection. 24WL = untransfected rats with 24 weeks of lesion. 12WL = 12 weeks after lesion. The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. One-way ANOVA and Newman-Keuls post-test. * <i>P</i> < 0.001, ** <i>P</i> < 0.0001. ns = no statistical significance, <i>P</i> > 0.05.</p>