Munc18 proteins bind non-cognate Sxs.
Michelle P. Christie
Shu-Hong Hu
Andrew E. Whitten
Asma Rehman
Russell J. Jarrott
Gordon J. King
Brett M. Collins
Jennifer L. Martin
10.1371/journal.pone.0187302.g002
https://plos.figshare.com/articles/figure/Munc18_proteins_bind_non-cognate_Sxs_/5557249
<p>Sx1<sub>1-261</sub>-His or Sx4<sub>1-275</sub>-His were incubated with detagged Munc18a or Munc18c for 2 h, 24 h or 48 h before the Sx was immobilized onto TALON<sup>™</sup> Co<sup>2+</sup> affinity beads for 1h and then washed. Samples of the beads were then run on SDS-PAGE and stained with Coomassie Blue to determine if detagged Munc18 had been pulled down by cognate and non-cognate Sx partners. Detagged Munc18a and Munc18c were also incubated for the same time periods on beads without bound Sx to monitor non-specific binding (control lanes). Solid vertical lines on the gel image denote the removal of intervening lanes or placing two different gels adjacent to each other.</p>
2017-10-31 17:47:43
non-cognate Munc 18c Syntaxin 1
Syntaxin 4. Munc 18a
Neuronal Munc 18a
SNARE protein Syntaxin
Revisiting interaction specificity
SNARE interaction specificity
Syntaxin N-peptide interaction
Munc 18c
Munc 18a
Regulatory Munc 18 proteins
SNARE proteins
partner SNARE Syntaxins
spatiotemporal fusion events
Munc 18c bind Syntaxins
SNARE partners SNAP 25
binding Syntaxin 4
binding interactions
adipocyte Munc 18 membrane fusion
Syntaxin 1 N-peptide
VAMP
Syntaxin 1
Syntaxin 4
adipocyte Munc 18c