Munc18 proteins bind non-cognate Sxs. Michelle P. Christie Shu-Hong Hu Andrew E. Whitten Asma Rehman Russell J. Jarrott Gordon J. King Brett M. Collins Jennifer L. Martin 10.1371/journal.pone.0187302.g002 https://plos.figshare.com/articles/figure/Munc18_proteins_bind_non-cognate_Sxs_/5557249 <p>Sx1<sub>1-261</sub>-His or Sx4<sub>1-275</sub>-His were incubated with detagged Munc18a or Munc18c for 2 h, 24 h or 48 h before the Sx was immobilized onto TALON<sup>™</sup> Co<sup>2+</sup> affinity beads for 1h and then washed. Samples of the beads were then run on SDS-PAGE and stained with Coomassie Blue to determine if detagged Munc18 had been pulled down by cognate and non-cognate Sx partners. Detagged Munc18a and Munc18c were also incubated for the same time periods on beads without bound Sx to monitor non-specific binding (control lanes). Solid vertical lines on the gel image denote the removal of intervening lanes or placing two different gels adjacent to each other.</p> 2017-10-31 17:47:43 non-cognate Munc 18c Syntaxin 1 Syntaxin 4. Munc 18a Neuronal Munc 18a SNARE protein Syntaxin Revisiting interaction specificity SNARE interaction specificity Syntaxin N-peptide interaction Munc 18c Munc 18a Regulatory Munc 18 proteins SNARE proteins partner SNARE Syntaxins spatiotemporal fusion events Munc 18c bind Syntaxins SNARE partners SNAP 25 binding Syntaxin 4 binding interactions adipocyte Munc 18 membrane fusion Syntaxin 1 N-peptide VAMP Syntaxin 1 Syntaxin 4 adipocyte Munc 18c