10.1371/journal.pone.0187302.g002
Michelle P. Christie
Michelle
P. Christie
Shu-Hong Hu
Shu-Hong
Hu
Andrew E. Whitten
Andrew E.
Whitten
Asma Rehman
Asma
Rehman
Russell J. Jarrott
Russell
J. Jarrott
Gordon J. King
Gordon
J. King
Brett M. Collins
Brett
M. Collins
Jennifer L. Martin
Jennifer L.
Martin
Munc18 proteins bind non-cognate Sxs.
Public Library of Science
2017
non-cognate Munc 18c Syntaxin 1
Syntaxin 4. Munc 18a
Neuronal Munc 18a
SNARE protein Syntaxin
Revisiting interaction specificity
SNARE interaction specificity
Syntaxin N-peptide interaction
Munc 18c
Munc 18a
Regulatory Munc 18 proteins
SNARE proteins
partner SNARE Syntaxins
spatiotemporal fusion events
Munc 18c bind Syntaxins
SNARE partners SNAP 25
binding Syntaxin 4
binding interactions
adipocyte Munc 18 membrane fusion
Syntaxin 1 N-peptide
VAMP
Syntaxin 1
Syntaxin 4
adipocyte Munc 18c
2017-10-31 17:47:43
Figure
https://plos.figshare.com/articles/figure/Munc18_proteins_bind_non-cognate_Sxs_/5557249
<p>Sx1<sub>1-261</sub>-His or Sx4<sub>1-275</sub>-His were incubated with detagged Munc18a or Munc18c for 2 h, 24 h or 48 h before the Sx was immobilized onto TALON<sup>™</sup> Co<sup>2+</sup> affinity beads for 1h and then washed. Samples of the beads were then run on SDS-PAGE and stained with Coomassie Blue to determine if detagged Munc18 had been pulled down by cognate and non-cognate Sx partners. Detagged Munc18a and Munc18c were also incubated for the same time periods on beads without bound Sx to monitor non-specific binding (control lanes). Solid vertical lines on the gel image denote the removal of intervening lanes or placing two different gels adjacent to each other.</p>