%0 Figure %A Ridge, Liam A. %A Mitchell, Karen %A Al-Anbaki, Ali %A Qureshi, Wasay Mohiuddin Shaikh %A Stephen, Louise A. %A Tenin, Gennadiy %A Lu, Yinhui %A Lupu, Irina-Elena %A Clowes, Christopher %A Robertson, Abigail %A Barnes, Emma %A A. Wright, Jayne %A Keavney, Bernard %A Ehler, Elisabeth %A C. Lovell, Simon %A E. Kadler, Karl %A E. Hentges, Kathryn %D 2017 %T EHC mutants display defects in cardiac development. %U https://plos.figshare.com/articles/figure/_i_EHC_i_mutants_display_defects_in_cardiac_development_/5550550 %R 10.1371/journal.pgen.1007068.g004 %2 https://plos.figshare.com/ndownloader/files/9790915 %K epicardial cell function %K encodes NMHC IIB %K vessel formation %K EHC epicardium displays %K EHC phenotype results %K NMHC IIB %K EMT %K Non-muscle myosin IIB %K ENU mutagenesis screen %K EPDC %K epicardial function %K splice donor site %K Myh 10 gene %K Myh 10 mutation causes %K defect %K candidate gene analysis %X

A: Heterozygous littermate control at E11.5 showing developing heart in pericardial sac. B: EHC mutant has pericardial effusion and blood visible in pericardial sac (arrow). C: Transverse section of control littermate at E11.5. The boxed region indicates the segment shown in panel C’. C’: Note the absence of blood cells on the cardiac surface and in the pericardial space. D: Transverse section of EHC mutant at E11.5 showing enlarged atria. The boxed region indicates the segment shown in panel D’. D’: Blood cells are present on the surface of the EHC heart (black arrow) and in the pericardial space (orange arrow). E: In heterozygous littermates at E14.5 the ventricular chamber is surrounded by a thick compact myocardium, which is highly organised. F: At E14.5, EHC mutants show a reduced compact ventricular myocardium (indicated by asterisk), accompanied by a thinning of the ventricular septal tissue (arrow). Note the abnormal atrial morphology and different localisation of the atria with respect to the ventricle compared to control (E). G: Heterozygous heart at E16.5 shows expected ventricular vascular connections and also coronary vessels on the cardiac surface (arrow). H: EHC mutant heart shows double-outlet right ventricle (DORV) (asterisk) as well as an absence of surface coronary vessels (arrow). Note the vesicle-like structures present on the ventricular surface. I: EHC/Myh10∆ and J: Myh10∆/Myh10∆ hearts at E16.5 similarly show DORV (asterisks) and ventricular vesicles (arrows). Scale bar = 200 μm (E, F), 1mm (A-D, G-J). Abbreviations: Het: heterozygote; lb: limb bud; LA: left atria, LV: left ventricle, RA: right atria, RV: right ventricle, VS: ventricular septum, ∆: Myh10∆.

%I PLOS Genetics