10.1371/journal.pone.0007234.g002
Elena Tassi
Elena
Tassi
Marco Braga
Marco
Braga
Renato Longhi
Renato
Longhi
Francesca Gavazzi
Francesca
Gavazzi
Giorgio Parmiani
Giorgio
Parmiani
Valerio Di Carlo
Valerio
Di Carlo
Maria Pia Protti
Maria
Pia Protti
Effect of titrated doses of IL-12 or IL-27 as single agent on the repertoire of cytokine secreted by CEA<sub>177–189/355–367</sub> specific CD4<sup>+</sup> T cells.
Public Library of Science
2013
titrated
doses
il-12
il-27
repertoire
cytokine
secreted
2013-02-21 04:19:54
Figure
https://plos.figshare.com/articles/figure/_Effect_of_titrated_doses_of_IL_12_or_IL_27_as_single_agent_on_the_repertoire_of_cytokine_secreted_by_CEA_177_8211_189_355_8211_367_specific_CD4_T_cells_/551225
<p><i>(A).</i> CD4<sup>+</sup> T cells were cultured with the relevant peptide and irradiated LCL as APC in the presence of increasing concentrations of IL-27 (0-0,1-1-10-100 ng/ml); after 2 days the cytokine release was evaluated by CBA (IL-5 IL-4, and IFN-γ) or ELISA (IL-13). The data for IL-13 are means of duplicate determination±SD. <i>(B).</i> CD4<sup>+</sup> T cells were cultured and tested, as described above, in the presence of increasing concentrations of IL-12 (0-5-20 ng/ml). The basal level of cytokines secretion of CD4<sup>+</sup> T cells in the presence of LCL only was subtracted from the sample values and was comprised between 0 and 0,018 ng/ml. The data are representative of at least three experiments.</p>