Raoufi-Rad, Newsha McRobb, Lucinda S. Lee, Vivienne S. Bervini, David Grace, Michael Ukath, Jaysree Mchattan, Joshua K. A. Sreenivasan, Varun Duong, T. T. Hong Zhao, Zhenjun Stoodley, Marcus A. ELISA and immunocytochemical analysis of ICAM-1 and VCAM-1 expression in irradiated bEnd.3 cells. <p>ELISA analysis of surface ICAM-1 (A) and VCAM-1 (D) expression in irradiated bEnd.3 cells normalized to Janus green absorbance to account for changes in cell number. Immunocytochemistry was performed on bEnd.3 cells using CF555-conjugated ICAM-1 or CF640-conjugated VCAM-1 antibodies (red). Staining was quantitated as integrated density using Image J (arbitrary units) for ICAM-1 (B) and VCAM-1 (E). Representative images are shown at 120 h (ICAM-1) (C) or 72 h (VCAM-1) (F) post-radiation at doses of 0–25 Gy. Isotype controls for ICAM-1 (IgG1-CF555) and VCAM-1 (IgG2b-CF640) showed no staining (representative images shown at 25 Gy, 72h). Cells were counterstained with DAPI to visualize nuclei (blue). All images were acquired at a magnification of 200× (scale bar = 100 μm). Values are mean ± SEM, n = 3 for each group.</p> AVM vessels;arteriovenous malformation Focussed radiosurgery;NIR;intercellular adhesion molecule 1;adhesion molecule expression;25 Gy;15 Gy dose;cell adhesion molecule 1;Gamma Knife surgery;ELISA;vivo;VCAM;rat AVM model;Cultured brain microvascular EC;ICAM;cell adhesion molecule expression 2017-09-26
    https://plos.figshare.com/articles/figure/ELISA_and_immunocytochemical_analysis_of_ICAM-1_and_VCAM-1_expression_in_irradiated_bEnd_3_cells_/5444812
10.1371/journal.pone.0185393.g003