10.1371/journal.pone.0185393.g003
Newsha Raoufi-Rad
Newsha
Raoufi-Rad
Lucinda S. McRobb
Lucinda S.
McRobb
Vivienne S. Lee
Vivienne S.
Lee
David Bervini
David
Bervini
Michael Grace
Michael
Grace
Jaysree Ukath
Jaysree
Ukath
Joshua Mchattan
Joshua
Mchattan
Varun K. A. Sreenivasan
Varun
K. A. Sreenivasan
T. T. Hong Duong
T. T. Hong
Duong
Zhenjun Zhao
Zhenjun
Zhao
Marcus A. Stoodley
Marcus A.
Stoodley
ELISA and immunocytochemical analysis of ICAM-1 and VCAM-1 expression in irradiated bEnd.3 cells.
Public Library of Science
2017
AVM vessels
arteriovenous malformation Focussed radiosurgery
NIR
intercellular adhesion molecule 1
adhesion molecule expression
25 Gy
15 Gy dose
cell adhesion molecule 1
Gamma Knife surgery
ELISA
vivo
VCAM
rat AVM model
Cultured brain microvascular EC
ICAM
cell adhesion molecule expression
2017-09-26 17:36:17
Figure
https://plos.figshare.com/articles/figure/ELISA_and_immunocytochemical_analysis_of_ICAM-1_and_VCAM-1_expression_in_irradiated_bEnd_3_cells_/5444812
<p>ELISA analysis of surface ICAM-1 (A) and VCAM-1 (D) expression in irradiated bEnd.3 cells normalized to Janus green absorbance to account for changes in cell number. Immunocytochemistry was performed on bEnd.3 cells using CF555-conjugated ICAM-1 or CF640-conjugated VCAM-1 antibodies (red). Staining was quantitated as integrated density using Image J (arbitrary units) for ICAM-1 (B) and VCAM-1 (E). Representative images are shown at 120 h (ICAM-1) (C) or 72 h (VCAM-1) (F) post-radiation at doses of 0–25 Gy. Isotype controls for ICAM-1 (IgG1-CF555) and VCAM-1 (IgG2b-CF640) showed no staining (representative images shown at 25 Gy, 72h). Cells were counterstained with DAPI to visualize nuclei (blue). All images were acquired at a magnification of 200× (scale bar = 100 μm). Values are mean ± SEM, n = 3 for each group.</p>