10.1371/journal.pone.0185393.g003 Newsha Raoufi-Rad Newsha Raoufi-Rad Lucinda S. McRobb Lucinda S. McRobb Vivienne S. Lee Vivienne S. Lee David Bervini David Bervini Michael Grace Michael Grace Jaysree Ukath Jaysree Ukath Joshua Mchattan Joshua Mchattan Varun K. A. Sreenivasan Varun K. A. Sreenivasan T. T. Hong Duong T. T. Hong Duong Zhenjun Zhao Zhenjun Zhao Marcus A. Stoodley Marcus A. Stoodley ELISA and immunocytochemical analysis of ICAM-1 and VCAM-1 expression in irradiated bEnd.3 cells. Public Library of Science 2017 AVM vessels arteriovenous malformation Focussed radiosurgery NIR intercellular adhesion molecule 1 adhesion molecule expression 25 Gy 15 Gy dose cell adhesion molecule 1 Gamma Knife surgery ELISA vivo VCAM rat AVM model Cultured brain microvascular EC ICAM cell adhesion molecule expression 2017-09-26 17:36:17 Figure https://plos.figshare.com/articles/figure/ELISA_and_immunocytochemical_analysis_of_ICAM-1_and_VCAM-1_expression_in_irradiated_bEnd_3_cells_/5444812 <p>ELISA analysis of surface ICAM-1 (A) and VCAM-1 (D) expression in irradiated bEnd.3 cells normalized to Janus green absorbance to account for changes in cell number. Immunocytochemistry was performed on bEnd.3 cells using CF555-conjugated ICAM-1 or CF640-conjugated VCAM-1 antibodies (red). Staining was quantitated as integrated density using Image J (arbitrary units) for ICAM-1 (B) and VCAM-1 (E). Representative images are shown at 120 h (ICAM-1) (C) or 72 h (VCAM-1) (F) post-radiation at doses of 0–25 Gy. Isotype controls for ICAM-1 (IgG1-CF555) and VCAM-1 (IgG2b-CF640) showed no staining (representative images shown at 25 Gy, 72h). Cells were counterstained with DAPI to visualize nuclei (blue). All images were acquired at a magnification of 200× (scale bar = 100 μm). Values are mean ± SEM, n = 3 for each group.</p>