Disruption of <i>PiT1</i> in MEFs does not affect Na<sup>+</sup>-Pi cotransport but leads to reduced proliferation. BeckLaurent LeroyChristine Beck-CormierSarah ForandAnne SalaünChristine ParisNadine BernierAdeline Ureña-TorresPablo PriéDominique OlleroMario CoulombelLaure FriedlanderGérard 2010 <p>(<i>A</i>) MEFs proliferation curve. <i>PiT1</i><sup><i>Δ</i><i>5/</i><i>Δ</i><i>5</i></sup> MEFs display a mean doubling time of 38 h, whereas <i>PiT1<sup>+/+</sup></i> and <i>PiT1</i><sup><i>Δ</i><i>5/+</i></sup> MEFs had a growth rate of 18 h and 20 h, respectively. (<i>B</i>) Na<sup>+</sup>-Pi uptake in MEFs. Disruption of <i>PiT1</i> in MEFs does not modify the overall Pi uptake. (<i>C</i>) Quantification of the expression of <i>PiT1</i> and <i>PiT2</i> mRNAs in <i>PiT1<sup>+/+</sup></i> and <i>PiT1</i><sup><i>Δ</i><i>5/</i><i>Δ</i><i>5</i></sup> MEFs by real-time RT-PCR. Note the 1.8-fold overexpression of <i>PiT2</i> mRNA in <i>PiT1</i>-null MEFs. * and # indicate significant differences as compared to wild-type controls with <i>P</i><0.05 and <i>P</i><0.001, respectively (Student's t test).</p>