Pathological changes (a,c) and immunohistochemical detection of viral antigen. Sharon M. Brookes Alejandro Núñez Bhudipa Choudhury Mikhail Matrosovich Stephen C. Essen Derek Clifford Marek J. Slomka Gaëlle Kuntz-Simon Fanny Garcon Bethany Nash Amanda Hanna Peter M. H. Heegaard Stéphane Quéguiner Chiara Chiapponi Michel Bublot Jaime Maldonado Garcia Rebecca Gardner Emanuela Foni Willie Loeffen Lars Larsen Kristien Van Reeth Jill Banks Richard M. Irvine Ian H. Brown 10.1371/journal.pone.0009068.g002 https://plos.figshare.com/articles/figure/_Pathological_changes_a_c_and_immunohistochemical_detection_of_viral_antigen_/534911 <p><b>a</b>. Lung. In-contact animal (TC4), 11 dpc. Multiple dark red areas of cranioventral pulmonary consolidation in the cranial and middle lung lobes. <b>b</b>. Nasal mucosa, 2 dpi. Influenza A nucleoprotein (brown) in numerous respiratory epithelial cells 20x. <b>c</b>. Lung, 7 dpi. Marked attenuation of bronchiolar epithelium due to epithelial cell necrosis, bronchiolar plug and peribronchiolar lymphohistiocytic infiltration. Haematoxylin and eosin 40x. <b>d</b>. Lung, 4 dpi. Viral nucleoprotein in abundant bronchiolar epithelial cells and cellular debris in the bronchiolar lumen and in several pulmonary alveolar macrophages 20x.</p> 2010-02-05 01:21:51 changes immunohistochemical detection viral