10.1371/journal.pone.0183764.g008
Miaorong Huang
Miaorong
Huang
Ruiai Chen
Ruiai
Chen
Guangcai Ren
Guangcai
Ren
Western blot analysis on purified rKer eluted from the Ni<sup>2+</sup>-NTA affinity chromatography.
Public Library of Science
2017
Bacillus licheniformis keratinase
peptide
sequence encoding Ker-His-Flag
coli DH 10Bac cell
insect cells system
pUC 57 vector
expression
Bacillus licheniformis PWD
DNA
sodium dodecyl sulfate polyacrylamide gel electrophoresis
NTA affinity chromatography
protein
8 His-Flag tag
insect cells
insect Spodoptera frugiperda
SDS-PAGE
2017-08-23 17:59:29
Figure
https://plos.figshare.com/articles/figure/Western_blot_analysis_on_purified_rKer_eluted_from_the_Ni_sup_2_sup_-NTA_affinity_chromatography_/5339116
<p>Lane 1, positive protein with Flag tag; Lane 2, cell lysates; Lane 3, cell supernatants and lane 4, purity. Signals of western blot were detected by a monoclonal antibody against C-terminus of Flag tag.</p>