10.1371/journal.pone.0183764.g008 Miaorong Huang Miaorong Huang Ruiai Chen Ruiai Chen Guangcai Ren Guangcai Ren Western blot analysis on purified rKer eluted from the Ni<sup>2+</sup>-NTA affinity chromatography. Public Library of Science 2017 Bacillus licheniformis keratinase peptide sequence encoding Ker-His-Flag coli DH 10Bac cell insect cells system pUC 57 vector expression Bacillus licheniformis PWD DNA sodium dodecyl sulfate polyacrylamide gel electrophoresis NTA affinity chromatography protein 8 His-Flag tag insect cells insect Spodoptera frugiperda SDS-PAGE 2017-08-23 17:59:29 Figure https://plos.figshare.com/articles/figure/Western_blot_analysis_on_purified_rKer_eluted_from_the_Ni_sup_2_sup_-NTA_affinity_chromatography_/5339116 <p>Lane 1, positive protein with Flag tag; Lane 2, cell lysates; Lane 3, cell supernatants and lane 4, purity. Signals of western blot were detected by a monoclonal antibody against C-terminus of Flag tag.</p>