10.1371/journal.pntd.0005805.g004
Janny A. Villa-Pulgarín
Janny A.
Villa-Pulgarín
Consuelo Gajate
Consuelo
Gajate
Javier Botet
Javier
Botet
Alberto Jimenez
Alberto
Jimenez
Nicole Justies
Nicole
Justies
Rubén E. Varela-M
Rubén
E. Varela-M
Álvaro Cuesta-Marbán
Álvaro
Cuesta-Marbán
Ingrid Müller
Ingrid
Müller
Manuel Modolell
Manuel
Modolell
José L. Revuelta
José L.
Revuelta
Faustino Mollinedo
Faustino
Mollinedo
Edelfosine induces breakage of kinetoplast DNA prior to nuclear DNA breakdown, and accumulates in mitochondria in <i>Leishmania</i> parasites and cancer cells.
Public Library of Science
2017
ATP synthase
lipid raft-located F O F 1
F O F 1
ATP synthase inhibition
Saccharomyces cerevisiae yeast
APL
tumor cells
tumor cell apoptosis-inducer edelfosine
edelfosine drug resistance
Leishmania axenic amastigotes
ether lipid edelfosine Background Leishmaniasis
DNA
novel druggable targets
Leishmania parasites
raft-located F O F 1
vivo antileishmanial activity
tumor cell mitochondria
edelfosine-induced cell death
intracellular Leishmania amastigotes
show antileishmanial activity
macrophage pro-inflammatory responses
2017-08-22 17:26:14
Figure
https://plos.figshare.com/articles/figure/Edelfosine_induces_breakage_of_kinetoplast_DNA_prior_to_nuclear_DNA_breakdown_and_accumulates_in_mitochondria_in_i_Leishmania_i_parasites_and_cancer_cells_/5332336
<p>(<b>A</b>) <i>L</i>. <i>panamensis</i> promastigotes were untreated (Control) or treated with 10 μM edelfosine (EDLF) for 6 and 9 h, and then analyzed by confocal microscopy for propidium iodide (PI) staining and TUNEL assay. The positions of the nucleus (N) and kinetoplast (K) are indicated by arrows. Merging of PI and TUNEL panels (Merge) shows the DNA-containing organelles with DNA disruption in yellow. The corresponding differential interference contrast (DIC) images were included in the Merge panels to highlight parasite morphology and facilitate kinetoplast identification. (<b>B</b>) <i>L</i>. <i>panamensis</i> promastigotes and (<b>C</b>) HeLa cancer cells were incubated with 10 μM PTE-ET (blue fluorescence) for 1 h, 100 nM MitoTracker (red fluorescence) for 20 min to localize mitochondria, and then analyzed by fluorescence microscopy. Areas of colocalization between mitochondria and PTE-ET in merge panels are purple. The corresponding differential interference contrast (DIC) images are also shown. Images are representative of three independent experiments. Bar, 20 μm.</p>