Common posterior Distal Tip Cell (DTC) trajectories in <i>ngat-1(ev821)</i> mutant animals.
Joseph Veyhl
Robert J. Dunn
Wendy L. Johnston
Alexa Bennett
Lijia W. Zhang
James W. Dennis
Harry Schachter
Joseph G. Culotti
10.1371/journal.pone.0183049.g001
https://plos.figshare.com/articles/figure/Common_posterior_Distal_Tip_Cell_DTC_trajectories_in_i_ngat-1_ev821_i_mutant_animals_/5322034
<p>Lateral view of the posterior gonad arm in L4 hermaphrodite animals by D.I.C. microscopy. In all panels, anterior is left, dorsal is up. (<b>A</b>) The 2 DTCs are born in ventral mid-body (in position of future vulva marked by triangle) and normally migrate post-embryonically in 3 phases [(1) thru (3)] to shape the developing U-shaped gonad arms. (<b>B</b>) Some <i>ngat-1(ev821)</i> animals grown at 25°C have a normal posterior DTC migration as in panel <b>A</b>, while many have an <i>unc-6</i> mutant-like phase 2 failure with phases 2 and 3 executed on the ventral side (panel <b>B</b>), while others (panel <b>C</b>) show a partial return to mid-body before migrating dorsally. To the right of panels <b>B</b> and <b>C</b> are the predicted corresponding ventral clear patch (VCP) phenotypes visible by low magnification stereomicroscopy (asterisk marks partial VCP in panel <b>C</b>). Scale bar in panel <b>A</b> (for <b>A-C</b>) is 50 μm.</p>
2017-08-17 17:57:39
DTC migration defects
phase 2 migration
stage DTC phase 2 migration errors
Caenorhabditis elegans
glycan-terminal GalNAc -β1-4GlcNAc
gly -13
gly -14
hermaphrodite Distal Tip Cell
penetrance ts phenotype
elegans gly -12
N-glycan
mutation
medial Golgi enzyme
DTC phase 2 migration
growth factor receptors
ts period
GnT 1 act
NGAT
DNA
Temperature shift analyses
ngat
phase 2 DTC pathfinding
stage-specific cell migration
phase migration pattern
modification
perinatal thermo-labile process