Common posterior Distal Tip Cell (DTC) trajectories in <i>ngat-1(ev821)</i> mutant animals. Joseph Veyhl Robert J. Dunn Wendy L. Johnston Alexa Bennett Lijia W. Zhang James W. Dennis Harry Schachter Joseph G. Culotti 10.1371/journal.pone.0183049.g001 https://plos.figshare.com/articles/figure/Common_posterior_Distal_Tip_Cell_DTC_trajectories_in_i_ngat-1_ev821_i_mutant_animals_/5322034 <p>Lateral view of the posterior gonad arm in L4 hermaphrodite animals by D.I.C. microscopy. In all panels, anterior is left, dorsal is up. (<b>A</b>) The 2 DTCs are born in ventral mid-body (in position of future vulva marked by triangle) and normally migrate post-embryonically in 3 phases [(1) thru (3)] to shape the developing U-shaped gonad arms. (<b>B</b>) Some <i>ngat-1(ev821)</i> animals grown at 25°C have a normal posterior DTC migration as in panel <b>A</b>, while many have an <i>unc-6</i> mutant-like phase 2 failure with phases 2 and 3 executed on the ventral side (panel <b>B</b>), while others (panel <b>C</b>) show a partial return to mid-body before migrating dorsally. To the right of panels <b>B</b> and <b>C</b> are the predicted corresponding ventral clear patch (VCP) phenotypes visible by low magnification stereomicroscopy (asterisk marks partial VCP in panel <b>C</b>). Scale bar in panel <b>A</b> (for <b>A-C</b>) is 50 μm.</p> 2017-08-17 17:57:39 DTC migration defects phase 2 migration stage DTC phase 2 migration errors Caenorhabditis elegans glycan-terminal GalNAc -β1-4GlcNAc gly -13 gly -14 hermaphrodite Distal Tip Cell penetrance ts phenotype elegans gly -12 N-glycan mutation medial Golgi enzyme DTC phase 2 migration growth factor receptors ts period GnT 1 act NGAT DNA Temperature shift analyses ngat phase 2 DTC pathfinding stage-specific cell migration phase migration pattern modification perinatal thermo-labile process