Comparison between pentaplex PCR and NCI panel of markers to determine MMR-deficiency in colorectal cancers. Ajay Goel Takeshi Nagasaka Richard Hamelin C. Richard Boland 10.1371/journal.pone.0009393.g004 https://plos.figshare.com/articles/figure/_Comparison_between_pentaplex_PCR_and_NCI_panel_of_markers_to_determine_MMR_deficiency_in_colorectal_cancers_/532108 <p><b>A</b>) The figure shows the performance comparison between the NCI panel markers and the QMVR optimized pentaplex PCR. Black squares indicate a tumor positive for allelic variation (or unstable) and white squares indicate a tumor negative for any allele variations (or stable). The dinucleotide repeat markers (D2S123, D5S346 and D17S250) are less robust than the mononucleotide repeats for detecting MSI. <b>B</b>) The figure illustrates the frequency of tumors with number of markers displaying allelic variations in MMR-deficient and proficient CRCs. As indicated, pentaplex PCR shows a higher sensitivity and specificity compared to NCI panel of markers, and the distribution of altered markers is unambiguously bimodal.</p> 2010-02-24 00:35:08 pentaplex pcr nci markers mmr-deficiency colorectal