10.1371/journal.pone.0009427.g003
Tsuyoshi Iwata
Tsuyoshi
Iwata
Denise Schultz
Denise
Schultz
Jessica Hicks
Jessica
Hicks
Gretchen K. Hubbard
Gretchen
K. Hubbard
Laura N. Mutton
Laura
N. Mutton
Tamara L. Lotan
Tamara
L. Lotan
Carlise Bethel
Carlise
Bethel
Matthew T. Lotz
Matthew
T. Lotz
Srinivasan Yegnasubramanian
Srinivasan
Yegnasubramanian
William G. Nelson
William
G. Nelson
Chi V. Dang
Chi
V. Dang
MengMeng Xu
MengMeng
Xu
Uzoma Anele
Uzoma
Anele
Cheryl M. Koh
Cheryl
M. Koh
Charles J. Bieberich
Charles J.
Bieberich
Angelo M. De Marzo
Angelo
M. De Marzo
MYC overexpression corresponds precisely to morphological changes in luminal cells that are characteristic of PIN.
Public Library of Science
2010
overexpression
corresponds
morphological
changes
luminal
cells
2010-02-25 00:35:03
Figure
https://plos.figshare.com/articles/figure/_MYC_overexpression_corresponds_precisely_to_morphological_changes_in_luminal_cells_that_are_characteristic_of_PIN_/532103
<p>(A–C). Low power (original magnification 20×) image of an entire lobe of a mouse ventral prostate at four weeks of age from a Lo-MYC mouse. Stains are indicated above images. Sections are not directly adjacent but are within ∼25–40 µM (of each other. Note the highly heterogeneous nature of the staining for MYC and Nkx3.1. (D–F). Medium power (100 ×) view of regions shown in the box in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0009427#pone-0009427-g003" target="_blank">Figure 3A</a>. Regions corresponding to normal appearing epithelium are circled in black, and regions corresponding to PIN lesions are circled in red. Note that regions of PIN are staining positively for MYC and the same regions are staining at a much reduced level for Nkx3.1. Also note small area in panel E that lacks staining for MYC, indicated by the arrow, which has strong positive staining for Nkx3.1 (indicated by the arrow in panel F). (G–I). Higher power image of region of transition between normal epithelium and PIN (the lowermost gland in panels D–F). Here it is clearly evident that cells corresponding to PIN cells, as shown in the H&E section (panel G) are the same cells that are staining strongly positive for MYC, and the same cells that have markedly reduced staining for Nkx3.1. (J) Western blot (left) showing reduced Nkx3.1 protein in a Lo-MYC mouse ventral prostate with PIN, as compared to an FVB wild-type mouse at the same age (six months). Northern blot (right) showing reduced <i>Nkx3.1</i> mRNA in a Lo-MYC mouse ventral prostate with PIN as compared to an FVB mouse at the same age (9 weeks). Top right shows <i>MYC</i> mRNA is highly elevated in another Lo-MYC mouse ventral prostate as compared to a matched FVB wild-type (9 weeks).</p>