%0 Figure %A Hung, Chi-Feng %A Hsiao, Chien-Yu %A Hsieh, Wen-Hao %A Li, Hsin-Ju %A Tsai, Yi-Ju %A Lin, Chun-Nan %A Chang, Hsun-Hsien %A Wu, Nan-Lin %D 2017 %T 18β-GA-d increased the phosphorylation of Akt, ERK1/2, and p38 in human dermal fibroblasts. %U https://plos.figshare.com/articles/figure/18_-GA-d_increased_the_phosphorylation_of_Akt_ERK1_2_and_p38_in_human_dermal_fibroblasts_/5315833 %R 10.1371/journal.pone.0182981.g005 %2 https://plos.figshare.com/ndownloader/files/9122062 %K HaCaT keratinocytes %K 18β- GA derivative-induced expression %K migration %K 18β- GA %K role %K aquaporin -3 expression %K aquaporin -3 %K proliferation %K fibroblast %K compound %K scratch wound healing assay %K ERK %K cause cell death %X

(A) Human dermal fibroblasts were treated with 0.1% THF or 18β-GA-d (30 μM) for different times. The level of phosphorylation of Akt, ERK1/2, and p38 at different times was analyzed by western blot assay. The quantification data for the ratios of phosphorylated protein/total non-phosphorylated protein in THF- and 18β-GA-d- treated groups to those in the untreated groups are shown in the lower panels. *p < 0.05 compared with THF treatment at the same time point (mean ± S.E.M. n = 3). (B) Human dermal fibroblasts were pretreated with UO126 (ERK inhibitor) (20 μM), MK2206 (Akt inhibitor) (5 μM), or SB203580 (p38 inhibitor) (10 μM) and then treated with 0.1% THF or 18β-GA-d (30μM) for 24 h. The protein expression of AQP-3 was evaluated by western blot assay. The quantification data for ratios of AQP-3/α-tubulin in different groups, relative to AQP-3/α-tubulin in the THF-treated group, are shown in the right panel. *p < 0.05 (mean ± S.E.M. n = 3).

%I PLOS ONE