Oxidative and glycolytic skeletal muscles show marked differences in gene expression profile in Chinese Qingyuan partridge chickens
Shu Jingting
Xiao Qin
Shan Yanju
Zhang Ming
Tu Yunjie
Ji Gaige
Sheng Zhongwei
Zou Jianmin
10.1371/journal.pone.0183118
https://plos.figshare.com/articles/dataset/Oxidative_and_glycolytic_skeletal_muscles_show_marked_differences_in_gene_expression_profile_in_Chinese_Qingyuan_partridge_chickens/5315803
<div><p>Oxidative and glycolytic myofibers have different structures and metabolic characteristics and their ratios are important in determining poultry meat quality. However, the molecular mechanisms underlying their differences are unclear. In this study, global gene expression profiling was conducted in oxidative skeletal muscle (obtained from the <i>soleus</i>, or SOL) and glycolytic skeletal muscle (obtained from the <i>extensor digitorum longus</i>, or EDL) of Chinese Qingyuan partridge chickens, using the Agilent Chicken Gene Expression Chip. A total of 1224 genes with at least 2-fold differences were identified (<i>P</i> < 0.05), of which 654 were upregulated and 570 were downregulated in SOL. GO, KEGG pathway, and co-expressed gene network analyses suggested that <i>PRKAG3</i>, <i>ATP2A2</i>, and <i>PPARGC1A</i> might play important roles in myofiber composition. The function of <i>PPARGC1A</i> gene was further validated. <i>PPARGC1A</i> mRNA expression levels were higher in SOL than in EDL muscles throughout the early postnatal development stages. In myoblast cells, shRNA knockdown of <i>PPARGC1A</i> significantly inhibited some muscle development and transition-related genes, including <i>PPP3CA</i>, <i>MEF2C</i>, and <i>SM</i> (<i>P</i> < 0.01 or <i>P</i> < 0.05), and significantly upregulated the expression of <i>FWM</i> (<i>P</i> < 0.05). Our study demonstrates strong transcriptome differences between oxidative and glycolytic myofibers, and the results suggest that <i>PPARGC1A</i> is a key gene involved in chicken myofiber composition and transition.</p></div>
2017-08-16 17:30:23
FWM
3CA
Chinese Qingyuan partridge chickens Oxidative
PRKAG
MEF
2A
2C
extensor digitorum longus
postnatal development stages
co-expressed gene network analyses
PPARGC 1A gene
Agilent Chicken Gene Expression Chip
ATP
KEGG
Chinese Qingyuan partridge chickens
EDL
SM
PPARGC 1A mRNA expression levels
glycolytic myofibers
poultry meat quality
SOL
PPP
chicken myofiber composition
gene expression profile
PPARGC 1A