%0 Figure %A M. Kouwenhoven, Willemieke %A von Oerthel, Lars %A P. Smidt, Marten %D 2017 %T Breeding schedule and outcome of the generation of En1KO;Pitx3GFP/GFP animals. %U https://plos.figshare.com/articles/figure/Breeding_schedule_and_outcome_of_the_generation_of_En1KO_Pitx3GFP_GFP_animals_/5304736 %R 10.1371/journal.pone.0182421.g001 %2 https://plos.figshare.com/ndownloader/files/9093673 %K ventral tegmental area %K En 1 tm 1Alj %K En 1 control %K 3KO %K programming mdDA neurons %K homeobox transcription factor Engrailed 1 %K En 1KO animals %K dopaminergic cell fate %K pool Mesodiencephalic dopaminergic %K En 1 %K VTA %K Ahd 2 expression %K mdDA neurons %K Pitx 3 %K paired-like homeodomain transcription factor 3 %K En 1 expression %K caudal subset identity %X

(A) En1Het;Pitx3GFP/GFP animals were inter-crossed to generate a litter that includes three genotypes: En1WT;Pitx3GFP/GFP, En1Het;Pitx3GFP/GFP, and En1KO;Pitx3GFP/GFP. (B) The presence of double En1KO;Pitx3GFP/GFP animals occurred below (Mendelian) predicted chance level (12.7% versus 25%). (C) DAPI staining at E14.5 reveals that in En1WT;Pitx3GFP/GFP and En1Het;Pitx3GFP/GFP animals the cerebellar anlage is correctly developed (Cb), whilst it is absent in the double En1KO;Pitx3GFP/GFP (*). (Rf: retroflexus, Cb: cerebellum, Cp: choroid plexus).

%I PLOS ONE