Bonaventura, Paola Lamboux, Aline Albarède, Francis Miossec, Pierre Zinc reverses the anti-proliferative and anti-inflammatory effects of Cd on synoviocytes. <p>(A,B) OA and RA synoviocytes were treated overnight with IL-17A (50 ng/mL) and TNF-α (0.5 ng/mL) followed by the addition of Zn, Cd or the combination of both. Cell viability and phase contrast microscopy photographs were assessed at day 5. (C) OA and RA synoviocytes were treated in normal cultures as in A or in co-cultures with inactivated or PHA-activated PBMCs. Supernatants were collected at day 8 and IL-6 production was quantified by ELISA. Statistical significance was assessed by a two-way Anova. Data are presented as mean ± SEM; * refers to the analysis of cytokine exposure, # to the analysis of metal exposure */# p<0.05, **/## p<0.01.</p> anti-inflammatory effects;Interleukin -17;Cd accumulation;RA;OA;anti-proliferative effects;Regulatory effects;-1X;MT -1s;gene expression;Rheumatoid arthritis;Cell viability;tissue inhibitor;inductively-coupled-plasma mass-spectrometry;cadmium-induced cytotoxicity;Cd-induced MT -1s expression;ELISA;mechanisms Zn;Results Synoviocytes;Conclusion Zinc counteracts;system activation;MMP;ZIP -8 expression;tumor necrosis factor-alpha;Cd anti-proliferative;ZIP -8 importer;intra-cellular content;TNF;inflammation Objectives Zinc;70 Zn spike;metal import;IL -6 production;metalloprotease -3 2017-07-25
    https://plos.figshare.com/articles/figure/Zinc_reverses_the_anti-proliferative_and_anti-inflammatory_effects_of_Cd_on_synoviocytes_/5244064
10.1371/journal.pone.0180879.g004