Vartanian, Vladimir Tumova, Jana Dobrzyn, Pawel Dobrzyn, Agnieszka Nakabeppu, Yusaku Lloyd, R. Stephen Sampath, Harini Mitochondrial DNA, protein, and gene expression. <p>Content of mtDNA (A) was measured by qPCR using targeted primers against regions encoding for ATP synthase F<sub>o</sub> subunit 6 (Atp6), Cytochrome c oxidase subunit 2 (CytoxII), and NADH dehydrogenase 5 (Nd5). Data are expressed as mean ± SEM for 6 animals in each genotype and are normalized for the high-copy number nuclear gene, 18S rRNA. Mitochondrial protein content (B) was measured by probing for proteins of oxidative phosphorylation (OXPHOS) and the voltage dependent anion channel (VDAC). Genes (C) and proteins (D) regulating mitochondrial fission, including DRP1 and FIS1 were measured by qPCR using gene-specific primers and by Western blotting, respectively. For protein gels, cropped images are presented for clarity. Full length blots are presented in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0181687#pone.0181687.s001" target="_blank">S1 Fig</a>. Data are representative of 4–6 animals per genotype and expressed as mean ± SEM; *p<0.05 vs. WT.</p> tissue function;lipid uptake;8- oxoguanine DNA glycosylase;OGG 1 gene product;tissue changes;muscle lipid deposition;treadmill endurance;muscle Oxidative stress;mitochondrial DNA;hepatic steatosis;muscle pathologies;muscle OGG 1;insulin resistance;oxidative DNA lesion;lipid metabolism;TCA cycle;base excision repair pathway;exogenous sources causes damage;mitochondrial fission;8- oxoguanine;OGG 1-deficient mice;grip strength;mitochondrial metabolism;OGG 1-deficient animals;DNA glycosylases;ring-fragmented purines;Oxidative DNA damage;high-fat diet-induced obesity;muscle function 2017-07-20
    https://plos.figshare.com/articles/figure/Mitochondrial_DNA_protein_and_gene_expression_/5229292
10.1371/journal.pone.0181687.g002