Jung, Sunhee Choi, Miri Choi, Kwangman Bin Kwon, Eun Kang, Mingu Kim, Dong-eun Jeong, Hyejeong Kim, Janghwan Heon Kim, Jong Kim, Mun Ock Han, Sang-Bae Cho, Sungchan Susceptibility of human DGAT2 activity to cysteine-specific modifying reagents. <p>(A) Membrane extracts from human DGAT2-overexpressing Sf9 insect cells were treated with indicated concentrations of NEM or IA. Human DGAT2 activity was measured by using the conventional extraction-based <i>in vitro</i> DGAT assay. The relative DGAT2 activity in percentage was calculated by setting the value from DMSO-treated sample to 100%. (B) Selective inhibitory effect of NEM on human DGAT2 activity compared to that on human DGAT1 and GPAT1. Membrane extracts from human DGAT2-, DGAT1-, or GPAT1-overexpressing Sf9 insect cells were treated with indicated concentrations of NEM or DMSO. Human DGAT1, DGAT2, and GPAT1 activity was measured by using the conventional extraction-based <i>in vitro</i> assays which are described in detail in the Materials and Methods section. The relative enzyme activity in percentage was calculated by setting the value from DMSO-treated sample to 100%. The mean values and standard deviations were determined from four independent assays.</p> DGAT 2;NEM;IA;DGAT 2 activity;DTT;disulfide crosslinking;ROS-related chemicals;role;GPAT;TG biosynthesis;Human DGAT 2 activity;cysteine residues Diacylglycerol acyltransferases;H 2 O 2 2017-07-11
    https://plos.figshare.com/articles/figure/Susceptibility_of_human_DGAT2_activity_to_cysteine-specific_modifying_reagents_/5196610
10.1371/journal.pone.0181076.g001