10.1371/journal.pone.0011182.g005
Sergio Roa
Sergio
Roa
Ziqiang Li
Ziqiang
Li
Jonathan U. Peled
Jonathan
U. Peled
Chunfang Zhao
Chunfang
Zhao
Winfried Edelmann
Winfried
Edelmann
Matthew D. Scharff
Matthew
D. Scharff
Model for MMR resolution of AID-generated dU:G mispairs.
Public Library of Science
2010
mmr
aid-generated
2010-06-17 01:34:49
Figure
https://plos.figshare.com/articles/figure/_Model_for_MMR_resolution_of_AID_generated_dU_G_mispairs_/515689
<p>In the DNA deamination model, MSH2/MSH6 recognizes the initial AID-generated dU:G lesion, the damaged strand is excised by Exo1, and Polη is preferentially recruited to the nontranscribed strand (NTS) to generate mutations at A sites within W<u>A</u> motifs. Fewer T sites within <u>T</u>W motifs are mutated, contributing to the singular A≫T and W<u>A</u>≫<u>T</u>W biases that characterize SHM and reflect the reduced targeting of A sites by Polη in the transcribed strand (TS). We highlight here the error-free repair effect of MMR at the dU:G mispairs within the excised patch, which is revealed in <i>Msh2<sup>−/−</sup>Msh6<sup>−/−</sup></i> mice by a significant increase in unrepaired dU:G lesions that are replicated as transitions at C:G sites in WR<u>C</u>/<u>G</u>YW hotspots (i.e. C-to-T within WR<u>C</u> in the NTS, and G-to-A within <u>G</u>YW as a reflection of C-to-T mutations in the TS).</p>