10.1371/journal.pone.0011177.g005
Benjamin Levi
Benjamin
Levi
Aaron W. James
Aaron
W. James
Emily R. Nelson
Emily
R. Nelson
Dean Vistnes
Dean
Vistnes
Benjamin Wu
Benjamin
Wu
Min Lee
Min
Lee
Ankur Gupta
Ankur
Gupta
Michael T. Longaker
Michael
T. Longaker
Human ASCs undergo osteogenic differentiation <i>in vivo</i>.
Public Library of Science
2010
ascs
osteogenic
differentiation
2010-06-17 01:34:41
Figure
https://plos.figshare.com/articles/figure/_Human_ASCs_undergo_osteogenic_differentiation_in_vivo_/515681
<p>Human ASCs transduced with a GFP encoding lentivirus were seeded in calvarial defects. (A) Histological analysis at 2 wks postoperative. The defect site (left) and contralateral aspect of the skull (right) from identical sections are imaged to show specificity of staining. (A, top) GFP immunohistochemistry, depicting evidence of human cells which appear brown. The contralateral side shows no GFP signal demonstrating the specificity of the stain. (A, middle) <i>Type I Collagen (COL1A1)</i> expression by <i>in situ</i> hybridization. (A, bottom) <i>Runt related transcription factor 2 (RUNX2)</i> expression by <i>in situ</i> hybridization. Staining can be seen in the uninjured contralateral periostium demonstrating specificity and can also be seen within the defect site. (B) Human osteogenic gene expression by qRT-PCR at 1 and 2 wks post-injury among hASC engrafted defects, including <i>hALP</i>, <i>hRUNX2</i>, *<i>P</i><0.01.</p>