10.1371/journal.pone.0011275.g004 Koji Sayama Koji Sayama Kentaro Kajiya Kentaro Kajiya Koji Sugawara Koji Sugawara Shintaro Sato Shintaro Sato Satoshi Hirakawa Satoshi Hirakawa Yuji Shirakata Yuji Shirakata Yasushi Hanakawa Yasushi Hanakawa Xiuju Dai Xiuju Dai Yumiko Ishimatsu-Tsuji Yumiko Ishimatsu-Tsuji Daniel Metzger Daniel Metzger Pierre Chambon Pierre Chambon Shizuo Akira Shizuo Akira Ralf Paus Ralf Paus Jiro Kishimoto Jiro Kishimoto Koji Hashimoto Koji Hashimoto Keratinocyte-specific TAK1 deletion delays hair cycle progression in adolescent mice. Public Library of Science 2013 tak1 deletion delays progression 2013-02-21 00:52:25 Figure https://plos.figshare.com/articles/figure/_Keratinocyte_specific_TAK1_deletion_delays_hair_cycle_progression_in_adolescent_mice_/514165 <p>(A) Schedule of tamoxifen application. The hair cycle was synchronized to anagen phase by wax depilation, and tamoxifen was topically applied to the dorsal skin of <i>Map3k7</i><sup>fl/fl</sup>K14-Cre-ER<sup>T2</sup>mice to delete TAK1. As controls, <i>Map3k7</i><sup>fl/fl</sup> mice or <i>Map3k7</i><sup>fl/fl</sup>K14-Cre-ER<sup>T2</sup> mice were treated with tamoxifen or ethanol, respectively. (B) Clinical appearance at the indicated time point after the depilation. At 2 weeks, hair shaft formation was noted in the control mice, while hair shaft growth was not seen in the tamoxifen-treated <i>Map3k7</i><sup>fl/fl</sup>K14-Cre-ER<sup>T2</sup> mice. (C) Histological analysis at the indicated time point after the depilation. Anagen progression was severely delayed in TAK1-deleted mice at 1–3 weeks. Scale bar, 100 µm.</p>