10.1371/journal.pone.0179633.g004 Katarzyna A. Mitręga Katarzyna A. Mitręga Adrianna M. Spałek Adrianna M. Spałek Jerzy Nożyński Jerzy Nożyński Maurycy Porc Maurycy Porc Magdalena Stankiewicz Magdalena Stankiewicz Tadeusz F. Krzemiński Tadeusz F. Krzemiński Representative histological findings of myocardial tissue evaluation at 35<sup>th</sup> day post-infarction after long-term oral treatment (6<sup>th</sup>– 35<sup>th</sup> day) with captopril (2 × 25 mg/kg) or M-2 (4 mg/kg) (hematoxylin and eozine-stained). Public Library of Science 2017 heart rate increase Cardiomyopathy development protection M -2 preload pressure M -2 therapy 25 years angiotensin-converting enzyme inhibitors heart tissue specimens captopril M -2. aortic pressure increase parameter flow 2017-06-21 17:43:12 Figure https://plos.figshare.com/articles/figure/Representative_histological_findings_of_myocardial_tissue_evaluation_at_35_sup_th_sup_day_post-infarction_after_long-term_oral_treatment_6_sup_th_sup_35_sup_th_sup_day_with_captopril_2_25_mg_kg_or_M-2_4_mg_kg_hematoxylin_and_eozine-stained_/5134189 <p>(A) Intact specimen of sham-operated heart, (B-C) Control 0.9% NaCl, (D) DMSO group, (E-F) Captopril group, (G) M-2 group (scanned with 20× objective, bar represents 50–100 μm). </p><p></p><p>(A)</p><p>Closely arranged cardiomyocytes with comparable nuclei. Normal myocardial tissue (magnification 30×).</p><p></p><p>(B)</p><p>Non-uniform cardiomyocytes with enlarged, rounded nuclei suggesting early cardiomyopathic process. Interstitial space contains some lymphocytes (magnification 30×).</p><p></p><p>(C)</p><p>Evident cardiomyopathic pattern with perivascular inflammatory infiltrations composed of lymphocytes and larger macrophages (magnification 30×).</p><p></p><p>(D)</p><p>Myocardial tissue morphology resembles intact group specimens (magnification 30×).</p><p></p><p>(E)</p><p>Typical cardiomyopathic pattern with numerous, dense inflammatory infiltrations with evident histiocytes, lymphocytes and other cells (magnification 30×).</p><p></p><p>(F)</p><p>Advanced cardiomyopathy with present fibrotic focai (magnification 20×).</p><p></p><p>(G)</p><p>Moderate cardiomyopathic pattern with lesser inflammatory infiltrations in comparison to captopril group. Regular fibrotic process (magnification 20×).</p><p></p><p></p> <p></p> <p>Closely arranged cardiomyocytes with comparable nuclei. Normal myocardial tissue (magnification 30×).</p> <p>Non-uniform cardiomyocytes with enlarged, rounded nuclei suggesting early cardiomyopathic process. Interstitial space contains some lymphocytes (magnification 30×).</p> <p>Evident cardiomyopathic pattern with perivascular inflammatory infiltrations composed of lymphocytes and larger macrophages (magnification 30×).</p> <p>Myocardial tissue morphology resembles intact group specimens (magnification 30×).</p> <p>Typical cardiomyopathic pattern with numerous, dense inflammatory infiltrations with evident histiocytes, lymphocytes and other cells (magnification 30×).</p> <p>Advanced cardiomyopathy with present fibrotic focai (magnification 20×).</p> <p>Moderate cardiomyopathic pattern with lesser inflammatory infiltrations in comparison to captopril group. Regular fibrotic process (magnification 20×).</p>