10.1371/journal.pbio.1000422.g006 Yoichiro Tamori Yoichiro Tamori Carl Uli Bialucha Carl Uli Bialucha Ai-Guo Tian Ai-Guo Tian Mihoko Kajita Mihoko Kajita Yi-Chun Huang Yi-Chun Huang Mark Norman Mark Norman Nicholas Harrison Nicholas Harrison John Poulton John Poulton Kenzo Ivanovitch Kenzo Ivanovitch Lena Disch Lena Disch Tao Liu Tao Liu Wu-Min Deng Wu-Min Deng Yasuyuki Fujita Yasuyuki Fujita Mahjong acts as a downstream target of Lgl in cell competition and basal cell extrusion. Public Library of Science 2010 acts downstream lgl basal 2010-07-13 00:23:46 Figure https://plos.figshare.com/articles/figure/_Mahjong_acts_as_a_downstream_target_of_Lgl_in_cell_competition_and_basal_cell_extrusion_/511426 <p>The mosaic analysis with a repressible cell marker (MARCM) system was used to overexpress UAS constructs in either <i>mahj</i><sup>−/−</sup> clones (A) or <i>lgl</i><sup>−/−</sup> clones (B, D, and E). Homozygous mutant clones are marked by the expression of GFP. (A) <i>mahj</i><sup>−/−</sup> MARCM clones overexpressing Lgl at 96 h after clone induction. (B, D, and E) <i>lgl</i><sup>−/−</sup> MARCM clones overexpressing Mahj at 144 h (B and D) or 96 h (E) after clone induction. (C) Quantification of apoptotic cells in the <i>lgl</i><sup>−/−</sup> mosaic wing discs with or without overexpression of Mahj. The percentage of apoptosis occurring in <i>lgl</i><sup>−/−</sup> cells on the boundary with wild-type cells was examined. The results represent means±SD (<i>n</i> = 13 discs, *<i>p</i><0.001). (D) A transverse section. Anti-cleaved Caspase-3 (A and B), anti-DE-Cadherin (D), and anti-phospho JNK (E) antibodies were used for immunostaining. (A, B, D, and E) Nuclei were stained with DAPI (blue).</p>