10.1371/journal.pone.0179421.g005 Lijun Zou Lijun Zou Xiaocheng Wang Xiaocheng Wang Liping Jiang Liping Jiang Shengping Wang Shengping Wang Xia Xiong Xia Xiong Huansheng Yang Huansheng Yang Wei Gao Wei Gao Min Gong Min Gong Chien-An A. Hu Chien-An A. Hu Yulong Yin Yulong Yin The roles of <i>FZD6</i> in cell viability of siRNA treated cells. Public Library of Science 2017 FZD 6 abundance Sus scrofa FZD 6 Sfz 6 mRNA Frizzled 6 crypt cells JNK Sfz 6 gene encodes Western blot analysis porcine enterocyte cells FZD 6 integral membrane protein FZD 6 protein signal transduction pathways PCP signal pathway components expression porcine FZD 6 gene IPEC-J 2 cells Nucleotide sequence analysis Tissue distribution analysis c-Jun N-terminal kinase 1 ras-related C 3 botulinum toxin substrate 1 2017-06-14 17:46:24 Figure https://plos.figshare.com/articles/figure/The_roles_of_i_FZD6_i_in_cell_viability_of_siRNA_treated_cells_/5109274 <p>(A) CCK-8 results of IPEC-J2 cells at different time points. NC = Negative Control; Data are expressed as the mean ±SEM, n = 6. (B) Flow cytometric analysis of Annexin V/PI in IPEC-J2 cells. IPEC-J2 cells were treated with 30nm, 50nm and 100nm <i>FZD6</i> siRNA-1 for 48h, respectively. Untreated cells were considered a negative control. (C) Relative apoptosis rate of cells in each group; Data are expressed as the mean ±SEM, n = 3. Values not sharing common letters are significantly different at <i>P</i> < 0.05.</p>