Western blotting results in each group. YangYue WangYang KongYawei ZhangXiaoning BaiLunhao 2017 <p>Effect of different frequency with same amount of treadmill exercise on the expression of MMP-13, TIMP-1, NF-κB p65, IκB-β, and COL2A1 expression in knee joint cartilage of rats with MIA-induced OA. Protein expression was determined in western blots of total protein extracted from cartilage tissues as described in Materials and Methods. The data was obtained in three separate experiments β-actin as an internal standard. Differences between CG and the OAG group were significant (*<i>P</i> < 0.001), and differences between the OAG and the treadmill frequency groups were significant (<sup>+</sup><i>P</i><0.001, <sup>+a</sup><i>P</i> = 0.030, <sup>+b</sup><i>P</i> = 0.001, <sup>+c</sup><i>P</i> = 0.039, <sup>+d</sup><i>P</i> = 0.010, <sup>+e</sup><i>P</i> = 0.038, <sup>+f</sup><i>P</i> = 0.002,). One-way ANOVA, n = 3 rats for each group, means with 95% confidence interval.</p>