Hsieh, Ying-hsin Huang, Ying-ju Zhang, Hao Liu, Qian Lu, Yang Yang, Hsiuchin Houghton, John Jiang, Chun Sui, Sen-Fang C. Tai, Phang Functional complementation of SecA fragments with C-terminal fragment C34. <p>(A) Channel activity in SecA domains reconstituted with C34, SecA<sub>610-901</sub>. (B) Translocation activity in SecA domains reconstituted with C34. N609 together with C34 have no activity, and could serve as internal negative controls. The reactions were conducted with liposomes with SecYEG or SecYEG-SecDFC as indicated. 100% translocation activity is SecA with SecYEG-DFC.</p> N 831. SecA domain fragments;SecA-only protein-conducting channels;ion channel activity;protein translocation capability;N-terminal fragment SecAN 493;N-terminal domain fragments;ATPase activity;form multi-functional domains;Sec-dependent translocation pathways;N 619-N Lipids;SecA domain fragment interactions;pore structure;form high-efficiency channels;C-terminal deletion fragments;protein translocation;non-functional N-terminal fragments;643-N;901 aminoacyl residues;SecYEG-SecDF;protein translocation ability;form SecA-only pore structures;amino-acyl residues SecA 619-831;fragments N 608-N;SecA form pore-ring structures;C-terminal fragments complement 2017-06-02
    https://plos.figshare.com/articles/figure/Functional_complementation_of_SecA_fragments_with_C-terminal_fragment_C34_/5068711
10.1371/journal.pone.0178307.g006