ARE reporter response in mesenchymal type cells. Waqas Azeem Margrete Reime Hellem Jan Roger Olsen Yaping Hua Kristo Marvyin Yi Qu Biaoyang Lin Xisong Ke Anne Margrete Øyan Karl-Henning Kalland 10.1371/journal.pone.0177861.g007 https://plos.figshare.com/articles/figure/ARE_reporter_response_in_mesenchymal_type_cells_/5059387 <p>(A) FACS analysis of mCherry signals in EPT3-PT1-AR-241B cells. Cells were grown in androgen free medium and treated with different AR agonists (1 nM R1881 and 1 μM DHT), steroids (10 nM dexamethasone, 10 nM progesterone and 1 μM β-estradiol), AR antagonist (10 μM enzalutamide) and anti-androgen (10 μM abiraterone). (B) Texas red (Tx-Red) indirect immunofluorescent detection of AR in EPT3-PT1-AR-241B cells. The cells were treated with ± 1 nM R1881 for 24 hours.</p> 2017-06-01 17:34:58 cell lines transduced luciferase reporter genes reporter activity LNCaP cells luminal differentiation transcriptome tumorigenic mesenchymal progeny cells androgen synthesis inhibitor lentiviral vector backbones epithelial cell lines prostate cells reporter vectors AR target gene activation background reporter activity mesenchymal type cells prostate cancer CRPC prostate cancer cells androgen receptor activity EP 156T cells androgen response element