ARE reporter response in mesenchymal type cells.
Waqas Azeem
Margrete Reime Hellem
Jan Roger Olsen
Yaping Hua
Kristo Marvyin
Yi Qu
Biaoyang Lin
Xisong Ke
Anne Margrete Øyan
Karl-Henning Kalland
10.1371/journal.pone.0177861.g007
https://plos.figshare.com/articles/figure/ARE_reporter_response_in_mesenchymal_type_cells_/5059387
<p>(A) FACS analysis of mCherry signals in EPT3-PT1-AR-241B cells. Cells were grown in androgen free medium and treated with different AR agonists (1 nM R1881 and 1 μM DHT), steroids (10 nM dexamethasone, 10 nM progesterone and 1 μM β-estradiol), AR antagonist (10 μM enzalutamide) and anti-androgen (10 μM abiraterone). (B) Texas red (Tx-Red) indirect immunofluorescent detection of AR in EPT3-PT1-AR-241B cells. The cells were treated with ± 1 nM R1881 for 24 hours.</p>
2017-06-01 17:34:58
cell lines transduced
luciferase reporter genes
reporter activity
LNCaP cells
luminal differentiation transcriptome
tumorigenic mesenchymal progeny cells
androgen synthesis inhibitor
lentiviral vector backbones
epithelial cell lines
prostate cells
reporter vectors
AR target gene activation
background reporter activity
mesenchymal type cells
prostate cancer
CRPC
prostate cancer cells
androgen receptor activity
EP 156T cells
androgen response element