%0 Figure %A P. Shanware, Naval %A Zhan, Lihong %A A. Hutchinson, John %A Hwa Kim, Sang %A M. Williams, Leah %A S. Tibbetts, Randal %D 2010 %T B56γ-PP2A mediates dephosphorylation of CREB and ATF1. %U https://plos.figshare.com/articles/figure/_B56_947_PP2A_mediates_dephosphorylation_of_CREB_and_ATF1_/505774 %R 10.1371/journal.pone.0012173.g003 %2 https://plos.figshare.com/ndownloader/files/835407 %K mediates %K dephosphorylation %K creb %X

(A) Okadaic acid (OA) sensitivity. HEK 293T cells were left untreated or exposed to 10 nM and 100 nM OA for 1 h. They were then subjected to IR for the indicated times. Cell extracts were prepared and analyzed by immunoblotting with α-CREB, α-pCREB-108/111/114 and α-pCREB-121 antibodies. (B) PP2Ac knockdown stimulates DNA damage-dependent CREB phosphorylation. HEK 293T cells expressing an shRNA targeting PP2Ac were compared to cells expressing a non-targeting shRNA construct. Cells were exposed to 10 Gy IR for 2 h and subjected to immunoblotting analysis with α-CREB, α-pCREB-108/111/114, α-pCREB-121 and α-PP2Ac antibodies. (C) B56γ knockdown stimulates DNA damage-dependent CREB phosphorylation. HEK 293T cells expressing an shRNA targeting B56γ were compared to cells expressing a non-targeting shRNA construct. Cells were exposed to 10 Gy IR for 1 h and subjected to immunoblotting analysis with α-CREB, α-pCREB-108/111/114, α-pCREB-121 and α-B56γ antibodies. (D) Effects of B56γ knockdown on ATF1 Ser-47/50/51 phosphorylation. HEK 293T cells were transiently transfected with control or B56γ siRNA and the levels of ATF1 Ser-47/50/51 phosphorylation assessed using α-pATF1-47/50/51 antibodies.

%I PLOS ONE