10.1371/journal.pone.0012393.g005
Eva Cernuda-Morollón
Eva
Cernuda-Morollón
Jaime Millán
Jaime
Millán
Mark Shipman
Mark
Shipman
Federica M. Marelli-Berg
Federica
M. Marelli-Berg
Anne J. Ridley
Anne
J. Ridley
TCR activation increases stathmin phosphorylation and alters microtubule organization.
Public Library of Science
2010
activation
increases
stathmin
phosphorylation
alters
microtubule
2010-08-25 01:19:56
Figure
https://plos.figshare.com/articles/figure/_TCR_activation_increases_stathmin_phosphorylation_and_alters_microtubule_organization_/504796
<p>T cells were plated on anti-CD3 antibody or control IgG for 45 min. (A) Cells were lysed and the levels of phospho-stathmin (pStathmin) and total stathmin assessed by western blotting. The graph shows relative levels of phospho-stathmin (normalized to total stathmin) of 3 independent experiments +/− S.E.M. in arbitrary units. (B) Antibody pre-treated T cells were collected, plated on ICAM-1 (IC-1) and lysed at the indicated time points. The levels of phospho-stathmin and total stathmin were assessed by western blotting. Representative blots are shown; the graph shows mean levels of phospho-stathmin (normalized to total stathmin) of 3 independent experiments +/− S.E.M. relative to IgG-treated cells at t = 0. (C) Antibody pre-treated cells expressing EB3-GFP were plated on ICAM-1-coated coverslips. Images were collected every 5 sec. Images shown correspond to five consecutive frames, from 130 sec after the start of the movie. Greyscale images (256 greyscale) were converted to indexed color (scale on right) to improve the visualization of small differences in intensity value. Arrows indicate individual microtubule tips moving towards the leading edge. Tracking images (right panels) show the movement of EB3-GFP in the 5 frames (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0012393#s4" target="_blank">Methods</a> for detail). Scale bars, 10 µm.</p>