10.1371/journal.pone.0177574.g001 Marco Fantini Marco Fantini Luca Pandolfini Luca Pandolfini Simonetta Lisi Simonetta Lisi Michele Chirichella Michele Chirichella Ivan Arisi Ivan Arisi Marco Terrigno Marco Terrigno Martina Goracci Martina Goracci Federico Cremisi Federico Cremisi Antonino Cattaneo Antonino Cattaneo Diagram of sequenced adaptor-antibody-adaptor constructs. Public Library of Science 2017 sample size Antibody Library sequencing error library elements intracellular protein interference studies Illumina platform antibody library complexity bioinformatic analysis antibody library antibody library complexity quality assessment error compensation Antibody libraries antibody library diversity antibody repertoires Inferring complexity Quantitative evaluation sequence antibody libraries NGS approach antibody library complexity estimate Diversity Estimator transformation efficiency generation sequencing Next-generation sequencing 2017-05-15 17:50:25 Figure https://plos.figshare.com/articles/figure/Diagram_of_sequenced_adaptor-antibody-adaptor_constructs_/5007176 <p>A) scFv library (gray), comprising heavy (VH) and light chain (VL) Complementary Determining Regions (CDR), was ligated to adapters (light green and pink) harbouring Illumina P5 and P7 flowcell hybridization sequences (green and red). B) VH nanobody library (gray), comprising heavy chain (VH) Complementary Determining Regions (CDR), was ligated to adapters (light green and pink) harbouring Illumina P5 and P7 flowcell hybridization sequences (green and red). The forward read (R1) uses SBS3 sequencing primer (Illumina), while the reverse read (R2) uses SBS12 primer (Illumina). iS1 and iS2 = index/shifter sequences.</p>