10.1371/journal.pone.0177574.g001
Marco Fantini
Marco
Fantini
Luca Pandolfini
Luca
Pandolfini
Simonetta Lisi
Simonetta
Lisi
Michele Chirichella
Michele
Chirichella
Ivan Arisi
Ivan
Arisi
Marco Terrigno
Marco
Terrigno
Martina Goracci
Martina
Goracci
Federico Cremisi
Federico
Cremisi
Antonino Cattaneo
Antonino
Cattaneo
Diagram of sequenced adaptor-antibody-adaptor constructs.
Public Library of Science
2017
sample size
Antibody Library
sequencing error
library elements
intracellular protein interference studies
Illumina platform
antibody library complexity
bioinformatic analysis
antibody library
antibody library complexity quality assessment
error compensation Antibody libraries
antibody library diversity
antibody repertoires
Inferring complexity
Quantitative evaluation
sequence antibody libraries
NGS approach
antibody library complexity estimate
Diversity Estimator
transformation efficiency
generation sequencing
Next-generation sequencing
2017-05-15 17:50:25
Figure
https://plos.figshare.com/articles/figure/Diagram_of_sequenced_adaptor-antibody-adaptor_constructs_/5007176
<p>A) scFv library (gray), comprising heavy (VH) and light chain (VL) Complementary Determining Regions (CDR), was ligated to adapters (light green and pink) harbouring Illumina P5 and P7 flowcell hybridization sequences (green and red). B) VH nanobody library (gray), comprising heavy chain (VH) Complementary Determining Regions (CDR), was ligated to adapters (light green and pink) harbouring Illumina P5 and P7 flowcell hybridization sequences (green and red). The forward read (R1) uses SBS3 sequencing primer (Illumina), while the reverse read (R2) uses SBS12 primer (Illumina). iS1 and iS2 = index/shifter sequences.</p>