%0 Figure %A Wang, Qian %A Xue, Huijun %A Li, Siqi %A Chen, Ying %A Tian, Xuelei %A Xu, Xin %A Xiao, Wei %A Fu, Yu Vincent %D 2017 %T The subcellular localizations of mCherry-Ubc13 and Rad5-sfGFP. %U https://plos.figshare.com/articles/figure/The_subcellular_localizations_of_mCherry-Ubc13_and_Rad5-sfGFP_/4958216 %R 10.1371/journal.pone.0176184.g005 %2 https://plos.figshare.com/ndownloader/files/8345792 %K selectable markers %K HA %K target genes %K genomic loci %K RAD 5 %K protein tag %K UBC 13 %K epitope tags %K tag HHT 1 %K epitope tag %K study target proteins %K protein fusion tags %K yeast Saccharomyces cerevisiae %K chromosomal location %K counter-selection system %K chromosomal locus %K target gene %X

HK578-10A (upper A) and HK578-10D (upper B) are the wild-type yeast strains without modification; the tagged strains PPGK1-Ubc13-mCherry and PPGK1-Rad5-sfGFP are shown at the bottom of A and bottom of B, respectively. The nucleus was stained by DAPI. The images were obtained under Plan-Apochromat 63×/1.40 oil (Zeiss LSM780).

%I PLOS ONE