PCR analysis of tag-<i>UBC13</i>/<i>RAD5</i> yeast strains. Qian Wang Huijun Xue Siqi Li Ying Chen Xuelei Tian Xin Xu Wei Xiao Yu Vincent Fu 10.1371/journal.pone.0176184.g004 https://plos.figshare.com/articles/figure/PCR_analysis_of_tag-_i_UBC13_i_i_RAD5_i_yeast_strains_/4958210 <p>(A) mCherry-Ubc13 strain. The left panel displays the genomic DNA PCR data. Lane 1: DNA ladder marker. Lane 2: the original strain HK578-10A. Lane 3: the pop-in strain CUC-Ubc13. Lane 4: the pop-out strain mCherry-Ubc13. The right panel represents the corresponding genomic organizations at the locus of <i>UBC13</i> in these three different strains. (B) Rad5-sfGFP strain. The original strain is HK578-10D. (C) Rad5-mKikGR strain. The original strain is HK578-10D. Arrows indicate the locations of primers. The detail information of primers could be found in the part of yeast strains check primers in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0176184#pone.0176184.s006" target="_blank">S3 Table</a>.</p> 2017-05-01 17:39:48 selectable markers HA target genes genomic loci RAD 5 protein tag UBC 13 epitope tags tag HHT 1 epitope tag study target proteins protein fusion tags yeast Saccharomyces cerevisiae chromosomal location counter-selection system chromosomal locus target gene