PCR analysis of tag-<i>UBC13</i>/<i>RAD5</i> yeast strains.
Qian Wang
Huijun Xue
Siqi Li
Ying Chen
Xuelei Tian
Xin Xu
Wei Xiao
Yu Vincent Fu
10.1371/journal.pone.0176184.g004
https://plos.figshare.com/articles/figure/PCR_analysis_of_tag-_i_UBC13_i_i_RAD5_i_yeast_strains_/4958210
<p>(A) mCherry-Ubc13 strain. The left panel displays the genomic DNA PCR data. Lane 1: DNA ladder marker. Lane 2: the original strain HK578-10A. Lane 3: the pop-in strain CUC-Ubc13. Lane 4: the pop-out strain mCherry-Ubc13. The right panel represents the corresponding genomic organizations at the locus of <i>UBC13</i> in these three different strains. (B) Rad5-sfGFP strain. The original strain is HK578-10D. (C) Rad5-mKikGR strain. The original strain is HK578-10D. Arrows indicate the locations of primers. The detail information of primers could be found in the part of yeast strains check primers in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0176184#pone.0176184.s006" target="_blank">S3 Table</a>.</p>
2017-05-01 17:39:48
selectable markers
HA
target genes
genomic loci
RAD 5
protein tag
UBC 13
epitope tags
tag HHT 1
epitope tag
study target proteins
protein fusion tags
yeast Saccharomyces cerevisiae
chromosomal location
counter-selection system
chromosomal locus
target gene