PCR analysis of tag-<i>HHT1</i> yeast strains and their subcellular localizations. Qian Wang Huijun Xue Siqi Li Ying Chen Xuelei Tian Xin Xu Wei Xiao Yu Vincent Fu 10.1371/journal.pone.0176184.g003 https://plos.figshare.com/articles/figure/PCR_analysis_of_tag-_i_HHT1_i_yeast_strains_and_their_subcellular_localizations_/4958201 <p>(A) HHT1-sfGFP strain. The genomic DNA PCR data shows in the left panel. Lane 1: DNA ladder marker. Lane 2: the original strain yRH182. Lane 3 and 4: the pop-in and pop-out strain. (B) The subcellular localizations of HHT1-sfGFP. The yRH182 (upper) is the wild-type yeast strains without modification; the tagged strains <i>HHT1</i>-sfGFP are shown at the bottom. The images were obtained under Plan-Apochromat 63×/1.40 oil (Zeiss 5 Live).</p> 2017-05-01 17:39:48 selectable markers HA target genes genomic loci RAD 5 protein tag UBC 13 epitope tags tag HHT 1 epitope tag study target proteins protein fusion tags yeast Saccharomyces cerevisiae chromosomal location counter-selection system chromosomal locus target gene