PCR analysis of tag-<i>HHT1</i> yeast strains and their subcellular localizations.
Qian Wang
Huijun Xue
Siqi Li
Ying Chen
Xuelei Tian
Xin Xu
Wei Xiao
Yu Vincent Fu
10.1371/journal.pone.0176184.g003
https://plos.figshare.com/articles/figure/PCR_analysis_of_tag-_i_HHT1_i_yeast_strains_and_their_subcellular_localizations_/4958201
<p>(A) HHT1-sfGFP strain. The genomic DNA PCR data shows in the left panel. Lane 1: DNA ladder marker. Lane 2: the original strain yRH182. Lane 3 and 4: the pop-in and pop-out strain. (B) The subcellular localizations of HHT1-sfGFP. The yRH182 (upper) is the wild-type yeast strains without modification; the tagged strains <i>HHT1</i>-sfGFP are shown at the bottom. The images were obtained under Plan-Apochromat 63×/1.40 oil (Zeiss 5 Live).</p>
2017-05-01 17:39:48
selectable markers
HA
target genes
genomic loci
RAD 5
protein tag
UBC 13
epitope tags
tag HHT 1
epitope tag
study target proteins
protein fusion tags
yeast Saccharomyces cerevisiae
chromosomal location
counter-selection system
chromosomal locus
target gene