%0 Figure %A Wen, Chongqing %A Wu, Liyou %A Qin, Yujia %A D. Van Nostrand, Joy %A Ning, Daliang %A Sun, Bo %A Xue, Kai %A Liu, Feifei %A Deng, Ye %A Liang, Yuting %A Zhou, Jizhong %D 2017 %T Overlap of OTUs generated using Uclust between/among technical replicates at different sequencing depth. %U https://plos.figshare.com/articles/figure/Overlap_of_OTUs_generated_using_Uclust_between_among_technical_replicates_at_different_sequencing_depth_/4951262 %R 10.1371/journal.pone.0176716.g002 %2 https://plos.figshare.com/ndownloader/files/8330813 %K treatment effects %K amplicon sequencing %K 16 S rRNA gene amplicons %K V 4 region %K 16 S rRNA gene %K UMI %K sequence abundance %K MiSeq %K 18 soil samples %K gene amplicon sequencing %K PCR %K replicate %K reproducibility %K Detrended correspondence analysis %K OTU sequence abundance %K OTU overlap %X

At a sequencing depth of about 30,000 reads, overlap of both two and three replicates was approaching a plateau when singletons were not removed.

%I PLOS ONE