10.1371/journal.pone.0176716.g002 Chongqing Wen Chongqing Wen Liyou Wu Liyou Wu Yujia Qin Yujia Qin Joy D. Van Nostrand Joy D. Van Nostrand Daliang Ning Daliang Ning Bo Sun Bo Sun Kai Xue Kai Xue Feifei Liu Feifei Liu Ye Deng Ye Deng Yuting Liang Yuting Liang Jizhong Zhou Jizhong Zhou Overlap of OTUs generated using Uclust between/among technical replicates at different sequencing depth. Public Library of Science 2017 treatment effects amplicon sequencing 16 S rRNA gene amplicons V 4 region 16 S rRNA gene UMI sequence abundance MiSeq 18 soil samples gene amplicon sequencing PCR replicate reproducibility Detrended correspondence analysis OTU sequence abundance OTU overlap 2017-04-28 17:28:04 Figure https://plos.figshare.com/articles/figure/Overlap_of_OTUs_generated_using_Uclust_between_among_technical_replicates_at_different_sequencing_depth_/4951262 <p>At a sequencing depth of about 30,000 reads, overlap of both two and three replicates was approaching a plateau when singletons were not removed.</p>