Pressure-overload induced concentric hypertrophy and reactive fibrosis. Vanessa Hertig Kim Tardif Marc Andre Meus Natacha Duquette Louis Villeneuve Fanny Toussaint Jonathan Ledoux Angelino Calderone 10.1371/journal.pone.0176147.g001 https://plos.figshare.com/articles/figure/Pressure-overload_induced_concentric_hypertrophy_and_reactive_fibrosis_/4927475 <p>(<b>Panel A)</b> Left ventricular (LV) wall thickness, circumference, <b>(Panel B</b>) interstitial and perivascular fibrosis as determined by Masson Trichrome staining were significantly increased in suprarenal abdominal aorta constricted rats (SAC) versus sham rats. Analysis was performed on a subpopulation of SAC (n = 3) and sham (n = 4) rats and (*) denotes <i>p</i><0.05 versus sham as determined by a student’s unpaired t-test.</p> 2017-04-27 19:20:39 nestin expression fibrotic rat heart collagen type calibre blood vessels muscle α- actin staining pro-fibrotic peptide growth factors collagen-expressing mesenchymal cells nestin protein levels filament protein nestin TGF -β 1 TGF -β 1 stimulation collagen-immunoreactive mesenchymal cells prolyl 4- hydroxylase pressure-overloaded heart phosphatidylinositol 3- kinase Nestin protein levels reactive fibrotic response II cells co-expressing nestin