Zwicker, Stephanie Hattinger, Eva Bureik, Daniela Batycka-Baran, Aleksandra Schmidt, Andreas Gerber, Peter-Arne Rothenfusser, Simon Gilliet, Michel Ruzicka, Thomas Wolf, Ronald IFNγ and cytosolic dsDNA regulate inflammatory caspase-5 and IL-1β release by epidermal keratinocytes. <p>A-B, Regulation of IL-1β and caspase-5 in Th1/Th17 cytokine-stimulated keratinocytes analyzed by RTqPCR and normalized to β-actin. Data represent mean + SEM, *, <i>p</i> < 0.05; **, <i>p</i> < 0.01; ***, <i>p</i> < 0.001, Student’s <i>t</i> test, n = 9. C, Keratinocytes stimulated with IFNγ, transfected with dsDNA [Poly(dA:dT)] ± DNase and the DNA-dependent IL-1β release was analyzed by ELISA. D, Representative immunoblotting of corresponding supernatants analyzed for DNA-dependent activation of caspase-5 (exposure times; caspase-5, 30s; cleaved caspase-5, 30min) compared to loading control (Ponceau staining), three independent experiments. E, Keratinocytes stimulated with IFNγ, transfected with dsDNA and indicated siRNA and the caspase-5 dependent IL-1β release was analyzed by ELISA. C, E, Data represent mean + SEM, **, <i>p</i> < 0.01; ***, <i>p</i> < 0.001, Student’s <i>t</i> test, n = 3–6.</p> disease-intrinsic danger signals psoriasin;antimicrobial peptide psoriasin;NLPR 1-dependent caspase;psoriatic skin lesions;Th 17-mediated skin autoinflammation;Th 17 micro-milieu;DNA;interleukin -17A synergistically;NLRP 1-dependent caspase;IL;keratinocyte;NLRP 1-inflammasome Anti-inflammatory vitamin D;S 100A 2017-04-19
    https://plos.figshare.com/articles/figure/IFN_and_cytosolic_dsDNA_regulate_inflammatory_caspase-5_and_IL-1_release_by_epidermal_keratinocytes_/4889753
10.1371/journal.pone.0175153.g002