%0 Figure %A Dickerson, Matthew T. %A Vierra, Nicholas C. %A Milian, Sarah C. %A K. Dadi, Prasanna %A A. Jacobson, David %D 2017 %T TALK-1 interacts with iOPN in a heterologous expression system (HEK293) and in primary mouse islets. %U https://plos.figshare.com/articles/figure/TALK-1_interacts_with_iOPN_in_a_heterologous_expression_system_HEK293_and_in_primary_mouse_islets_/4870136 %R 10.1371/journal.pone.0175069.g001 %2 https://plos.figshare.com/ndownloader/files/8153888 %K TALK -1. %K β- cell function %K iOPN %K membrane-specific yeast two-hybrid %K MYTH %K type -2 diabetes %K HEK 293 cells %K Intracellular OPN %K K 2P channels %K TALK -1-interacting proteins %K islet cDNA library interacted %K KCNK %K control β- cell TALK %K transcript variant 2 channels %K β- cell TALK %K GSIS %K transcript variant 3 channels %K β- cells %K K 2P channel %K V m hyperpolarization %K diabetes-associated potassium channel TALK %X

A. Western blot run with TALK-1-FLAG immune complexes (isolated from HEK293 cells expressing iOPN-V5 and TALK-1-FLAG) probed with anti-V5. B. Western blot run with TALK-1 immune complexes (isolated from primary human islets) probed with anti-OPN. Immunofluorescent image of a HEK293 cell coexpressing C. OPN-V5 (red) and D. TALK-1-FLAG (green). E. Overlay of C. (OPN-V5) and D. (TALK-1-FLAG) demonstrating colocalization when heterologously expressed in HEK293 cells. Immunofluorescent image of a human pancreas section with F. endogenous OPN (red) and G. endogenous TALK-1 (green). H. Overlay of F. (OPN) and G. (TALK-1) showing islet specific colocalization (yellow).

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