%0 Figure %A Wang, Jiyong %A Hu, Qiwen %A Chen, Huijie %A Zhou, Zhipeng %A Li, Weihua %A Wang, Ying %A Li, Shaojie %A He, Qun %D 2010 %T DCAF11 levels drastically reduced in all csn mutants, possibly because of rapid autoubiquitination-mediated degradation. %U https://plos.figshare.com/articles/figure/_DCAF11_levels_drastically_reduced_in_all_csn_mutants_possibly_because_of_rapid_autoubiquitination_mediated_degradation_/486064 %R 10.1371/journal.pgen.1001232.g008 %2 https://plos.figshare.com/ndownloader/files/815703 %K levels %K drastically %K reduced %K autoubiquitination-mediated %X

(A) Immunoprecipitation assay confirming the interaction between Flag-Cul4 and Myc-DCAF11. Wild-type strain and wild-type strain expressing Myc-DCAF11 were used as negative controls. (B) Western blot analyses showing the degradation of Myc-DCAF11 in csn mutants after the addition of CHX. (C) Amino acid alignment of conserved WDXR motifs of DCAF11 homologs. (D) Immunoprecipitation assay with c-Myc antibody showing that Myc-DCAF11R432A failed to interact with Flag-DDB1. (E) Myc immunoprecipitation assay showing that Myc-DCAF11R432A failed to interact with Flag-Cul4. (F) Degradation of Myc-DCAF11R432A expressed in the wild-type strain and all csn mutants after the addition of CHX. Densitometric analyses from four independent experiments showing degradation of DCAF11 (G) and DCAF11R432A (H).

%I PLOS Genetics