10.1371/journal.pgen.1001232.g008
Jiyong Wang
Jiyong
Wang
Qiwen Hu
Qiwen
Hu
Huijie Chen
Huijie
Chen
Zhipeng Zhou
Zhipeng
Zhou
Weihua Li
Weihua
Li
Ying Wang
Ying
Wang
Shaojie Li
Shaojie
Li
Qun He
Qun
He
DCAF11 levels drastically reduced in all <i>csn</i> mutants, possibly because of rapid autoubiquitination-mediated degradation.
Public Library of Science
2010
levels
drastically
reduced
autoubiquitination-mediated
2010-12-02 01:41:04
Figure
https://plos.figshare.com/articles/figure/_DCAF11_levels_drastically_reduced_in_all_csn_mutants_possibly_because_of_rapid_autoubiquitination_mediated_degradation_/486064
<p>(A) Immunoprecipitation assay confirming the interaction between Flag-Cul4 and Myc-DCAF11. Wild-type strain and wild-type strain expressing Myc-DCAF11 were used as negative controls. (B) Western blot analyses showing the degradation of Myc-DCAF11 in <i>csn</i> mutants after the addition of CHX. (C) Amino acid alignment of conserved WDXR motifs of DCAF11 homologs. (D) Immunoprecipitation assay with c-Myc antibody showing that Myc-DCAF11R432A failed to interact with Flag-DDB1. (E) Myc immunoprecipitation assay showing that Myc-DCAF11R432A failed to interact with Flag-Cul4. (F) Degradation of Myc-DCAF11R432A expressed in the wild-type strain and all <i>csn</i> mutants after the addition of CHX. Densitometric analyses from four independent experiments showing degradation of DCAF11 (G) and DCAF11R432A (H).</p>