10.1371/journal.pgen.1001232.g008 Jiyong Wang Jiyong Wang Qiwen Hu Qiwen Hu Huijie Chen Huijie Chen Zhipeng Zhou Zhipeng Zhou Weihua Li Weihua Li Ying Wang Ying Wang Shaojie Li Shaojie Li Qun He Qun He DCAF11 levels drastically reduced in all <i>csn</i> mutants, possibly because of rapid autoubiquitination-mediated degradation. Public Library of Science 2010 levels drastically reduced autoubiquitination-mediated 2010-12-02 01:41:04 Figure https://plos.figshare.com/articles/figure/_DCAF11_levels_drastically_reduced_in_all_csn_mutants_possibly_because_of_rapid_autoubiquitination_mediated_degradation_/486064 <p>(A) Immunoprecipitation assay confirming the interaction between Flag-Cul4 and Myc-DCAF11. Wild-type strain and wild-type strain expressing Myc-DCAF11 were used as negative controls. (B) Western blot analyses showing the degradation of Myc-DCAF11 in <i>csn</i> mutants after the addition of CHX. (C) Amino acid alignment of conserved WDXR motifs of DCAF11 homologs. (D) Immunoprecipitation assay with c-Myc antibody showing that Myc-DCAF11R432A failed to interact with Flag-DDB1. (E) Myc immunoprecipitation assay showing that Myc-DCAF11R432A failed to interact with Flag-Cul4. (F) Degradation of Myc-DCAF11R432A expressed in the wild-type strain and all <i>csn</i> mutants after the addition of CHX. Densitometric analyses from four independent experiments showing degradation of DCAF11 (G) and DCAF11R432A (H).</p>