%0 Figure %A I. Povarova, Olga %A Kuznetsova, Irina M. %A Turoverov, Konstantin K. %D 2013 %T Actin aggregation induced by GdnHCl in low concentrations. %U https://plos.figshare.com/articles/figure/_Actin_aggregation_induced_by_GdnHCl_in_low_concentrations_/486012 %R 10.1371/journal.pone.0015035.g001 %2 https://plos.figshare.com/ndownloader/files/815644 %K aggregation %K induced %K gdnhcl %X

The dependence of ANS fluorescence intensity (A) and of light scattering (B) of the solutions of inactivated actin (red) and initially native actin after 10 min and 24 h of incubation in a solution of denaturants at the appropriate concentration (blue and green) on GdnHCl (closed symbols) and urea (open symbols) concentration. Insert in panel A. Scheme of actin denaturation and aggregation (N, U* and I are native, essential unfolded and inactivated actin [11], Iag is aggregates of inactivated actin, the details are given in the text). Insert in panel B. The dependence of the total macromolecule charge of actin on the pH of a solution calculated on the basis of protein amino acid content [24]. The protein concentration was 0.15 mg/ml, ANS concentration was 5⋅10−5 M.

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