Zimmermann, Michael Atmanene, Cédric Xu, Qingyan Fouillen, Laetitia Dorsselaer, Alain Van Bonnet, Dominique Marsol, Claire Hibert, Marcel Sanglier-Cianferani, Sarah Pigault, Claire K. McNamara, Laurie Watterson, D. Martin Haiech, Jacques Kilhoffer, Marie-Claude Ability of the fluorescent probe to monitor dimerization of DAPK catalytic core. <p>A) Chemical structure of CHPO 187-3 H11-para, which was selected as best hit in the primary and secondary screening assays (absorption and fluorescent spectra of the probe are provided in Supporting Information, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0014120#pone.0014120.s001" target="_blank">Figure S1</a>). B) Binding of CHPO 187-3 H11-para (0.1 µM) to DAPKwt at different concentrations of NH<sub>4</sub>Ac (• 5 mM, ▪ 100 mM and ▴ 250 mM). Fluorescence polarization (mFP) is plotted <i>versus</i> protein concentration. mFP corresponds to the polarization degree of the fluorescent probe ×10<sup>3</sup>. Error bars indicate the standard deviation of four independent titrations. C) Same than in B) but with DAPKdel.</p> fluorescent;probe;dimerization;dapk;catalytic 2010-11-30
    https://plos.figshare.com/articles/figure/_Ability_of_the_fluorescent_probe_to_monitor_dimerization_of_DAPK_catalytic_core_/485565
10.1371/journal.pone.0014120.g002