%0 Figure %A Selles, Benjamin %A Zannini, Flavien %A Couturier, Jérémy %A Jacquot, Jean-Pierre %A Rouhier, Nicolas %D 2017 %T Reduction of the PDI-M catalytic domains by AtNTRB as assayed using DTNB and insulin. %U https://plos.figshare.com/articles/figure/Reduction_of_the_PDI-M_catalytic_domains_by_AtNTRB_as_assayed_using_DTNB_and_insulin_/4808884 %R 10.1371/journal.pone.0174753.g007 %2 https://plos.figshare.com/ndownloader/files/7950913 %K Fe 2 S 2 %K reduction reactions %K -170 mV %K module %K Atypical protein disulfide isomerases %K NADPH thioredoxin reductase %K pH 7.0. %K oxidation %K WCKHC %K PDI-L 1A Protein disulfide isomerases %K non-physiological substrate NADP-MDH %K b domains influence %K multi-modular redox catalysts %K poplar PDI isoforms %K dithiol-disulfide exchange reactions %K PDI-L 1a %K Escherichia coli expression %K PDI-A %K PDI-M %K NADP malate dehydrogenase %X

A. DTNB reduction assay. Representative kinetic curves obtained in the presence of NADPH and AtNTRB alone or with 0.2 μM PDI-M, PDI-M() or PDI-M(a). B. Insulin reduction assessed using NADPH/AtNTRB as an electron donor system and 5 μM of PDI-M, PDI-M() or PDI-M(a).

%I PLOS ONE