Reduced lung viral titers of challenged IFN-α/βR<sup>−/−</sup> mice that received passive transfer of serum eluate and purified IgG from MuV-IA-immunized mice. Adrian Pickar Pei Xu Andrew Elson James Zengel Christian Sauder Steve Rubin Biao He 10.1371/journal.pone.0174444.g004 https://plos.figshare.com/articles/figure/Reduced_lung_viral_titers_of_challenged_IFN-_R_sup__sup_mice_that_received_passive_transfer_of_serum_eluate_and_purified_IgG_from_MuV-IA-immunized_mice_/4808398 <p>BALB/c mice were immunized i.n. with 1x10<sup>6</sup> PFU of MuV-IA in a volume of 100 μL. Mice were boosted at 3 and 5 wpi, and serum was collected and pooled at 8 wpi. IgG was purified by affinity chromatography as described in materials and methods and dialyzed in PBS. Column eluate (referred to as serum eluate) was collected during IgG purification. Naïve IFN-α/βR<sup>−/−</sup> mice received passive transfer i.p. of 1 mL PBS, serum eluate, or purified IgG. Mice were challenged 24 hours post passive transfer i.n. with 1x10<sup>6</sup> PFU of MuV-IA in a volume of 100 μL. Lungs were collected and homogenized 2 dpi and viral titers were determined by plaque assay in duplicate. The limit of detection of 5.4 PFU/g tissue is represented by the dashed line. ANOVA and Tukey multiple comparison tests were used to calculate <i>P</i> values.</p> 2017-03-31 18:06:40 mumps immunity IFN mumps virus infection outbreak animal model mumps virus