PS2 protein is increased in microglia activated by IFNγ. Suman Jayadev Amanda Case Alison J. Eastman Huy Nguyen Julia Pollak Jesse C. Wiley Thomas Möller Richard S. Morrison Gwenn A. Garden 10.1371/journal.pone.0015743.g005 https://plos.figshare.com/articles/figure/_PS2_protein_is_increased_in_microglia_activated_by_IFN_947_/480312 <p>A) Western blot analysis demonstrating the PS2 C-terminus migrating at ∼20 kDa. Twenty-four hour stimulation with 10 u/ml IFNγ induces increased levels of PS2 expression in lysates prepared from primary microglia, while 100 ng/ml LPS does not induce PS2 expression. B) Quantitative densitometry on Western blots of lysates from 4 independent cultures shows significantly increased induction of PS2 by IFNγ. Data represent the mean ± SEM of 4 independent experiments (*p<0.05; Student's t-test). C) Twenty-four hour stimulation of primary microglia with IFNγ results in significantly increased PS2 mRNA. Data represent the mean ± SEM of 3 independent experiments (** p<0.01 by Student's t-test).</p> 2010-12-29 00:05:12 microglia activated