10.1371/journal.pone.0015743.g005 Suman Jayadev Suman Jayadev Amanda Case Amanda Case Alison J. Eastman Alison J. Eastman Huy Nguyen Huy Nguyen Julia Pollak Julia Pollak Jesse C. Wiley Jesse C. Wiley Thomas Möller Thomas Möller Richard S. Morrison Richard S. Morrison Gwenn A. Garden Gwenn A. Garden PS2 protein is increased in microglia activated by IFNγ. Public Library of Science 2010 microglia activated 2010-12-29 00:05:12 Figure https://plos.figshare.com/articles/figure/_PS2_protein_is_increased_in_microglia_activated_by_IFN_947_/480312 <p>A) Western blot analysis demonstrating the PS2 C-terminus migrating at ∼20 kDa. Twenty-four hour stimulation with 10 u/ml IFNγ induces increased levels of PS2 expression in lysates prepared from primary microglia, while 100 ng/ml LPS does not induce PS2 expression. B) Quantitative densitometry on Western blots of lysates from 4 independent cultures shows significantly increased induction of PS2 by IFNγ. Data represent the mean ± SEM of 4 independent experiments (*p<0.05; Student's t-test). C) Twenty-four hour stimulation of primary microglia with IFNγ results in significantly increased PS2 mRNA. Data represent the mean ± SEM of 3 independent experiments (** p<0.01 by Student's t-test).</p>