10.1371/journal.pone.0174078.g002 Violeta Georgeta Trusca Violeta Georgeta Trusca Elena Valeria Fuior Elena Valeria Fuior Ioana Madalina Fenyo Ioana Madalina Fenyo Dimitris Kardassis Dimitris Kardassis Maya Simionescu Maya Simionescu Anca Violeta Gafencu Anca Violeta Gafencu <i>In vivo</i> cell-specific effect of dexamethasone on apoE expression in macrophages and liver of C57BL/6J mice. Public Library of Science 2017 apoE gene expression apoE promoter transcriptional activity influence apoE expression GR apoE promoter RAW 264.7 cells apoE expression hepatocytes Apolipoprotein E promoter deletion mutants apolipoprotein E gene expression apoE mRNA levels glucocorticoid HepG 2 cells RAW 264.7 macrophages PLA protein-DNA interaction assays C 57BL mice MEK 2017-03-29 17:37:05 Figure https://plos.figshare.com/articles/figure/_i_In_vivo_i_cell-specific_effect_of_dexamethasone_on_apoE_expression_in_macrophages_and_liver_of_C57BL_6J_mice_/4799179 <p>Representative images show the expression of apoE and GAPDH genes in mouse peritoneal macrophages <b>(A)</b> and liver extracts <b>(B)</b>, as detected by RT-PCR (upper panels). Quantification of apoE gene expression normalized to GAPDH in peritoneal macrophages and liver extracts in dexamethasone-treated mice, as compared to control mice, is depicted in the lower panels. Five-day dexamethasone (30mg/kg body weight) treatment of C57BL/6J mice significantly increases apoE mRNA levels in mouse peritoneal macrophages (p<0.001), but does not affect hepatic apoE gene expression (p>0.05).</p>