10.1371/journal.pone.0174078.g002
Violeta Georgeta Trusca
Violeta Georgeta
Trusca
Elena Valeria Fuior
Elena Valeria
Fuior
Ioana Madalina Fenyo
Ioana Madalina
Fenyo
Dimitris Kardassis
Dimitris
Kardassis
Maya Simionescu
Maya
Simionescu
Anca Violeta Gafencu
Anca Violeta
Gafencu
<i>In vivo</i> cell-specific effect of dexamethasone on apoE expression in macrophages and liver of C57BL/6J mice.
Public Library of Science
2017
apoE gene expression
apoE promoter transcriptional activity
influence apoE expression
GR
apoE promoter
RAW 264.7 cells
apoE expression
hepatocytes Apolipoprotein E
promoter deletion mutants
apolipoprotein E gene expression
apoE mRNA levels
glucocorticoid
HepG 2 cells
RAW 264.7 macrophages
PLA
protein-DNA interaction assays
C 57BL mice
MEK
2017-03-29 17:37:05
Figure
https://plos.figshare.com/articles/figure/_i_In_vivo_i_cell-specific_effect_of_dexamethasone_on_apoE_expression_in_macrophages_and_liver_of_C57BL_6J_mice_/4799179
<p>Representative images show the expression of apoE and GAPDH genes in mouse peritoneal macrophages <b>(A)</b> and liver extracts <b>(B)</b>, as detected by RT-PCR (upper panels). Quantification of apoE gene expression normalized to GAPDH in peritoneal macrophages and liver extracts in dexamethasone-treated mice, as compared to control mice, is depicted in the lower panels. Five-day dexamethasone (30mg/kg body weight) treatment of C57BL/6J mice significantly increases apoE mRNA levels in mouse peritoneal macrophages (p<0.001), but does not affect hepatic apoE gene expression (p>0.05).</p>